红花黄色素中主要成分的含量测定

目的 对药食两用天然色素红花黄色素进行质量控制。方法 采用紫外分光光度法和高效液相色谱法，以总黄色素和羟基红花黄色素A为指标，对红花黄色素中主要化学成分进行含量测定。结果 自制红花黄色素与市售红花黄色素(色价E=150)的总黄色素含量均＞99%，自制红花黄色素的羟基红花黄色素A含量最高，达20.21%，为市售红花黄色素的2~3倍。结论 市售红花黄色素的总黄色素含量随色价的增大而显著提高，不同的红花黄色素样品中羟基红花黄色素A的含量也存在较大差异，本研究为红花黄色素的质量分析控制奠定了基础。

Determination of Main Ingredients in Safflower Yellow

OBJECTIVE To study the quality of safflower yellow preliminarily which was medicine and food of natural pigments. METHODS The content of the total safflower yellow and the hydroxy safflower yellow A in the safflower yellow were determined by UV and HPLC. RESULTS The total content of the homemade safflower yellow and the safflower yellow (E=150) sold in the market were more than 99%. The content of hydroxyl safflower yellow A extracted from homemade safflower yellow was much higher, reached 20.21%, 2-3 times of the sold. CONCLUSION The content of safflower yellow increases remarkably with increase of the colourity, and the content of hydroxy safflower yellow A varies in different samples of safflower yellow. The research sets up a base for the analysis and the control of safflower yellow quality.