HPLC同时测定大豆磷脂中磷脂酰胆碱和溶血磷脂酰胆碱含量

目的建立同时测定大豆磷脂中磷脂酰胆碱（PC）和溶血磷脂酰胆碱（LPC）含量的方法。方法采用高效液相色谱法,色谱柱为Phenomenex silica柱（4.6 mm×150 mm,5μm）,流动相为正己烷-异丙醇-水（5∶80∶20）,流速：0.7 mL.min-1,检测波长：206 nm,柱温：25℃。结果 PC在0.4~2.4 mg.mL-1内呈良好的线性关系,平均回收率为99.4%,RSD为0.40%（n=6）;LPC在10~60μg.mL-1内呈良好的线性关系,平均回收率为98.0%,RSD为1.65%（n=6）。结论本试验方法简便、重复性好、专属性强,可作为大豆磷脂中PC,LPC的检测方法。

Determination of Phosphatidylcholine and Lysophosphatidylcholine in Soybean Phospholipids by HPLC

OBJECTIVE To establish an HPLC method for simultaneously determining phosphatidylcholine (PC), lysophosphatidylcholine (LPC) in soybean phospholipids. METHODS The HPLC system consisted of a Phenomenex silica column(4.6 mm×150 mm, 5 μm), a mobile phase of n-hexane-isopropanol-water(5∶80∶20). The flow rate was 0.7 mL.min-1. Column temperature was set at 25 ℃ and the wavelength of detector was set at 206 nm. RESULTS The calibration curve was linear in the range of 0.4-2.4 mg.mL-1 for PC and 10-60 μg.mL-1 for LPC, respectively. The average recovery for PC was 99.4% and RSD was 0.40%(n=6). The average recovery for LPC was 98.0% and RSD was 1.65%(n=6). CONCLUSION The method is simple, rapid and specially. It can be used for the determination of PC and LPC in soybean phospholipids.