温通针法对血管性痴呆大鼠脑组织SOD、MDA及AChE的影响

目的:观察温通针法对血管性痴呆(VD)模型大鼠脑组织内超氧化物歧化酶(SOD)、丙二醛(MDA)及乙酰胆碱酯酶(AChE)的影响,并探讨其相关机制。方法:将50只健康Wistar大鼠随机分为正常组、模型组、药物组、捻转针法组和温通针法组,采用反复夹闭双侧颈总动脉-再灌注方法造成血管性痴呆大鼠模型。针刺选穴为“大椎”“百会”“水沟”,分别施以温通或捻转手法;药物使用尼莫地平,0.0108 g/kg灌胃给药。治疗均每日1次,连续15 d。用比色法检测大鼠脑内MDA含量及SOD、AChE活性。结果:血管性痴呆模型组大鼠SOD活性较正常组显著降低(P<0.01),AChE活性、MDA含量较正常组皆显著升高(P<0.01);温通针法组、捻转针法组和药物组分别与模型组比较,均能明显升高SOD活性(P<0.01),降低AChE活性、MDA含量(P<0.05);温通针法组与药物组相比,升高SOD和降低AChE活性无显著性差异(P>0.05),降低MDA含量有显著差异(P<0.05);且温通针法组升高SOD活性,降低AChE活性、MDA含量的作用优于捻转针法组(P<0.05)。结论:温通针法具有抗脂质过氧化损伤及改善脑内胆碱能系统的能力。

Effect of Warmth-promotion Needling on Cerebral SOD, MDA and AChE in Vascular Dementia Rats

Objective: To observe the effect of warmth-promotion needling on cerebral malondialdehyde(MDA) content,superoxide dismutase(SOD) and acetylcholinesterase(AChE) activity so as to explore its underlying mechanism in improving vascular dementia(VD).Methods: Forty Wistar rats were randomized into control,model,medication,needle-twirling and warmth-promotion groups with 8 cases in each.VD model was established by occlusion of the bilateral common carotid arteries and reperfusion in combination with intraperitoneal injection of sodium nitroprusside(2.5 mg/kg).Warmth-promotion needling was applied to "Dazhui"(GV 14),"Baihui"(GV 20) and "Shuigou"(GV 26).Rats of medication group were intragastric perfusion of Nimodipine suspension(0.0108 g/kg).The treatment was given once daily continuously for 15 days.SOD and AChE activity and MDA content of the brain(right side) tissue were detected with purine oxidase method,hydroxylamine chromatometry and thio-malonylurea method separately.Results: In comparison with control group,SOD activity of model group decreased significantly(P<0.01),AChE activity and MDA content increased considerably (P<0.01);while compared with model group,SOD activity of warmth-promotion,needle-twirling and medication groups raised evidently(P<0.01),AChE activity and MDA content of the later 3 groups lowered significantly(P<0.05,0.01).The effects of warmth-promotion group were significantly superior to that of medication group in lowering MDA content and to those of needle-twirling group in raising SOD activity and lowering AChE activity and MDA content(P<0.05).Conclusion: Warmth-promotion needling can function well in resisting lipid peroxidation injury and lowering AChE activity in VD rats,which may contribute to its effect in improving learning-memory ability.