| 高效液相色谱法测定罗红霉素胶囊中罗红霉素的质量 |
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| 引用本文： | 喻樊,施文君.高效液相色谱法测定罗红霉素胶囊中罗红霉素的质量[J].抗感染药学,2010,7(3):191-193. |
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| 作者姓名： | 喻樊 施文君 |
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| 作者单位： | 盐城师范学院,江苏,盐城,224051 |
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| 基金项目： | 江苏省滩涂生物资源与环境保护重点建设实验室项目，盐城师范学院自然科学基金项目 |
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| 摘 要： | 目的:建立罗红霉素胶囊中罗红霉素质量的高效液相色谱法测定方法。方法:采用ODSC18柱(250mm×6mm,5μm),以0.1mol/L磷酸二氢铵溶液(三乙胺调节pH6.5)-乙腈(65∶35)为流动相,流速为1.0mL/min,检测波长210nm,进样量20μL。结果:罗红霉素在质量浓度10～80μg/mL的范围内与峰面积响应值呈良好线性关系(r=0.9997,n=5),平均回收率为99.84%,RSD=0.65%(n=6)。结论:该法操作简便,数据准确、可靠,适用于罗红霉素胶囊中罗红霉素质量的控制。
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| 关 键 词： | 罗红霉素胶囊 罗红霉素 质量 高效液相色谱法 |
Determination of Roxithromycin in Roxithromycin Capsules by HPLC |
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| YU Fan,SHI Wen-jun.Determination of Roxithromycin in Roxithromycin Capsules by HPLC[J].Anti-infection Pharmacy,2010,7(3):191-193. |
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| Authors: | YU Fan SHI Wen-jun |
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| Institution: | Yancheng Teachers College,Yancheng Jiangsu 224051,China |
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| Abstract: | Objective：To establish the determination method of roxithromycin in roxithromycin capsules by HPLC.Methods：ODS C18 column（250 mm×6 mm,5 μm）was applied,using 0.1 mol/L ammonium dihydrogen phosphate solution（adjusted pH to 6.5 with trithylamine）-acetonitrile（65：35）as mobile phase.The flow rate was 1.0 mL/min,the detection wavelength was at 210 nm and the injection volume was 20 μL.Results：The good linearity in the range of 10～80 μg/mL was showed（r = 0.999 7,n = 5）,and its average recovery rate was 99.84%,RSD = 0.65%（n = 6）.Conclusion：The method was rapid,accurate and reliable.It can be used for the content determination of roxithromycin capsules. |
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| Keywords: | roxithromycin capsules Roxithromycin Content HPLC |
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