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| 铁皮石斛组培品与野生品的形态组织学和多糖含量比较研究 | |
| 引用本文： | 范俊安,王继生,张艳,任凌燕,邱宗荫,夏永鹏. 铁皮石斛组培品与野生品的形态组织学和多糖含量比较研究[J]. 中国中药杂志, 2005, 30(21): 1648-1650 |
| 作者姓名： | 范俊安 王继生 张艳 任凌燕 邱宗荫 夏永鹏 |
| 作者单位： | 1. 重庆医科大学药学系,重庆,400016;重庆市食品药品监督管理局,重庆,400042;重庆市中药研究院,重庆,400065 2. 重庆医科大学药学系,重庆,400016 3. 重庆市中药研究院,重庆,400065 4. 重庆医科大学药学系,重庆,400016;重庆市中药研究院,重庆,400065 5. 重庆市食品药品监督管理局,重庆,400042 |
| 基金项目： | 重庆市科技攻关项目（01-6609-2） |
| 摘 要： | 目的:比较铁皮石斛组培品和野生品在植物形态、组织结构及总多糖含量上有无差异。方法:显微切片观察、显微摄影;苯酚-硫酸法测定多糖含量。结果与结论:铁皮石斛组培品与野生品在植物形态及组织结构上基本相同,总多糖含量无显著差异,表明铁皮石斛经组织培养和人工培植过程后药材性状和质量与野生药材相近,初步证明经过组织培养快速繁殖和人工培植铁皮石斛是解决其资源紧缺问题的有效途径。 |
| 关 键 词： | 铁皮石斛 组织培养 形态组织学 多糖 |
| 文章编号： | 1001-5302（2005）21-1648-03 |
| 收稿时间： | 2005-01-11 |
| 修稿时间： | 2005-01-11 |
A comparison of tissue formation and the content of polysaccharide between wild and cultured Dendrobium candidum |
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| FAN Jun-an;WANG Ji-sheng;ZHANG Yan;REN Ling-yan;QIU Zong-ying;XIA Yong-peng. A comparison of tissue formation and the content of polysaccharide between wild and cultured Dendrobium candidum[J]. China Journal of Chinese Materia Medica, 2005, 30(21): 1648-1650 | |
| Authors: | FAN Jun-an WANG Ji-sheng ZHANG Yan REN Ling-yan QIU Zong-ying XIA Yong-peng |
| Affiliation: | 1. Chongqing University of Medical Science, Chongqing 400016, Ch/na ; 2. Chongqing Food and Drug Administration, Chongqing 400042, China; 3. Chongqing Academy of Chinese Materia Medica , Chongqing 400065, China |
| Abstract: | Objecetive: To compare the tissue formation and the content of polysaccharide between the wild Dendrobium candidum and the cultured ones and to find any existed differences.Method: Bare-handed microtomy and photomicrography;The content of polysaccharide is determined by phenol-sulphuric acid method.Result and conclusion: There are no marked noticeable differences between the wild D.candidum and the cultured ones in terms of the tissure formation and the content of polysaccharide. |
| Keywords: | Dendrobium candidum tissue cultivation formation polysaccharide |
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