丙氨酰谷氨酰胺对脓毒症大鼠肺损伤的保护作用

目的：探讨丙氨酰谷氨酰胺对脓毒症大鼠急性肺损伤的保护作用及其机制。方法：静脉注射内毒素（LPS）3mL（6mg／kg）复制大鼠脓毒症急性肺损伤模型，40只健康Wistar大鼠，分为4组，即对照组（静脉注射等量生理盐水28mL／kg）、内毒素组[先后静脉注射生理盐水25mL／kg和内毒素3mL／kg（6mg／kg）]、丙氨酰谷氨酰胺治疗组[先后静脉注射4．5％力太（丙氨酰谷氨酰胺注射液）25mL/kg和内毒素3mL／kg（6mg／kg）]、谷氨酰胺治疗组[先后静脉注射3％谷氨酰胺25mL／kg和内毒素3mL／kg（6mg／kg）]。在静脉注射前（T0），注射后6h（T1），各抽血1mL。注射6h后，处死大鼠，用EusA法测定各时点血清TNF-α，IL-1β，IL-8的浓度，测定肺组织湿／干重比、支气管肺泡灌洗液的总蛋白浓度，光学显微镜下观察肺组织病理改变，原位TUNEL技术进行肺组织细胞凋亡检测分析。结果：与LPs组比较，丙氨酰谷氨酰胺治疗组、谷氨酰胺治疗组的肺组织湿／干重比、支气管肺泡灌洗液的总蛋白浓度，血清TNF-α，IL-1β，IL-8浓度明显降低（P＜0．05）；丙氨酰谷氨酰胺治疗组、谷氨酰胺治疗组的肺组织损伤程度明显减轻而且凋亡指数也较LPS组显著降低（P＜0．05）。结论：静脉给予丙氨酰谷氨酰胺对脓毒症大鼠急性肺损伤有保护作用。

Protective effects of alanyl-glutamine on acute lung injury induced by lipopolysaccharide in rats

Objective To investigate the effect of alanyl-glutamine(Ala-Gln) on acute lung injury(ALI) in rats induced by sepsis and its mechanisms.MethodsForty healthy adult Wistar rats were randomly divided into a control group,a lipopolysaccharide(LPS)-induced shock group,an Ala-Gln treated group,and a glutamine(Gln) treated group.The control group received an intravenous infusion of 28 mL/kg lactated Ringer's solution(LR).The LPS-induced shock group received an intravenous administration of 25 mL/kg LR,and then 3 m...