VA与VE对梭曼（GD）染毒大鼠MDA和MAO的影响

目的研究梭曼染毒大鼠丙二醛（MDA）含量和单胺氧化酶（MAO）活性的变化,探讨抗氧化剂VA,VE 对梭曼染毒大鼠自由基损伤的保护作用. 方法雄性大鼠 40只,按体质量随机分为4组：阴性对照组,阳性对照组,VA和VE实验组. 观察染毒大鼠血清、大脑、肝、肾组织MDA 含量和MAO活性的变化. 结果梭曼(GD)染毒大鼠阳性对照组5阴性5对照组相比较,脑组织MDA含量和MAO活性有显著性增加（P<0.05）;血清蛋白有显著减少（P<0.05）,肝蛋白有显著增加（ P<0.05）. VA实验组与阳性对照组比较,脑组织MDA有显著增加（P<0.01）,肝组织MDA有显著降低（P< 0.01）;脑组织MAO活性增加,但差异不显著. VE实验组与阳性对照组相比,肾脏MDA有显著减少（P<0.0 1）;脑组织MAO活性有非常显著的降低（P<0.01）. 施加VA, VE 抗氧化剂后,与阳性对照组比较,血清和肝蛋白有非常显著减少（P<0.01）,脑蛋白有显著减少（P<0.05）. 结论梭曼(GD)染毒引起明显脂质过氧化损伤,VE具有抗氧化保护作用,而VA有协同促进MDA升高的过氧化损伤作用.

Effects of vitamin A and vitamin E on malondialdehyde and mon oamine in poisoning rat by soman (GD)

AIM To study the changes of malondialdehyde content and monoamine (MAO) activity in rat injured by Soman (GD), and the antioxidation effect of vitamin A(V A) and vitamin E (V E). METHODS Forty male rats were randomly divided into 4 groups: negative group, positive group, V A and V E trail group. After normally fed for 10 d, GD is injected into muscle of rates of positive control group and V A, V E trail group. After toxic symptoms occurred in rate of positive group and V A, V E trail group partly for 2 h, the rats were killed by dislocation of head. MDA content and MAO activity of rat's serum, cerebrum, liver, kidney were determined respectively. RESULTS compared with negative control group, content of MDA and activity of MAO increase significantly in cerebrum ( P <0.05), concentration of serum protein increase and that of live protein decrease obviously ( P <0.05) in positive control group; in V A trial, content of MDA in cerebrum increase obviously ( P <0.01), but that of MDA in kidney decrease apparently ( P <0.01), activity of MAO have rarely increase. in V E trial, content of MDA in kidney decrease apparently ( P < 0.01), and activity of MAO reduce significantly ( P <0.01); in V A and V E trial, concentration of serum and live protein decrease remarkably ( P <0.01), that of cerebrum protein lessen apparently ( P <0.05). CONCLUSION GD raised lipid superoxidation, and V E decease obviously free radical injury,but V A accelerate the injury of free radical intoxicated rat.