早产鼠高氧肺损伤中肺泡内IL-8的含量变化

目的探讨早产鼠高氧肺损伤时肺泡内白介素8（IL-8）的含量变化。方法生后6 h的SD早产鼠120只,随机分为空气组和高氧组,每组60只。空气组常规饲养,高氧组置常压高氧箱内,吸入氧体积分数〉900 ml/L,两组分别于实验进行24 h、3 d、5 d、10 d、14 d取肺组织观察病理变化,用双抗体夹心法检测肺泡灌洗液（BALF）中IL-8含量。结果空气组随着日龄增加肺泡结构逐渐分化;高氧组3 d始见小血管充血扩张,红细胞渗出至肺泡腔,肺泡及间质见炎性细胞浸润;随着吸氧时间延长,肺发育受阻,肺泡结构紊乱,肺泡数量减少。从第3天起,BALF中IL-8含量显著增高,与相应同日龄空气组比较,差异有统计学意义（P均〈0.01）。结论 IL-8参与了高氧肺损伤的发生发展过程,是导致高氧肺损伤的重要炎症介质。

Changes of IL-8 Levels in Alveolar of Hyperoxic Lung Injury in Premature Rats

Objective To investigate the content of interleukin-8（IL-8）in pulmonary alveoli in preterm rats with hyperoxia-induced lung injury.Methods 120 preterm SD rats（6 hours after delivery）were divided randomly into two group： hyperoxia group（n=60）and air group（n=60）.Pups in hyperoxia group were exposed to hyperoxia（FiO2900 ml/L）and those in air group were raised normally inhaling room air.Histological changes of the lungs were observed and the contents of IL-8 in bronchoalveolar lavage fluid（BALF）were measured by ELISA on day 1,3,5,10,14,respectively.Results The structure of pulmonary alveoli developed gradually with aged in air group.Vasodilatation and engorgement in small vessels,red blood cell in alveolar space and inflammatory cell infiltration in alveolar space and interstitial could be found from day 3 in hyperoxia group.Along with prolong exposure to hyperoxia,lung development was impaired and alveoli structure appeared reduction in the number of alveolar.IL-8 level in BALF increased significantly in hyperoxia group from day 3 to day 14 compared to age-matched,air group（P0.01 in each matched groups）.Conclusion As an important inflammatory mediator,IL-8 contribute to the development of hyperoxia-induced lung injury.