甘草总黄酮的大孔吸附树脂纯化工艺优选

目的:优选大孔吸附树脂分离、纯化甘草总黄酮的工艺条件.方法:以总黄酮吸附量和解吸率为考察指标,对12种不同类型树脂进行筛选,在此基础上对其纯化工艺条件进行优选.结果:AB-8型大孔吸附树脂对甘草总黄酮的纯化效果最好,其最佳工艺条件为上样液质量浓度为0.2 g·mL-1,每mL树脂最大上样量为2 mL,吸附流速0.5 BV·h-1,上样液pH 6,6BV水以流速1 BV·h-1洗脱除杂,4 BV 70％乙醇以1 BV·h-1流速洗脱.该优选条件下,洗脱物中甘草总黄酮纯度为38％.结论:该优选工艺合理、可行,适合工业生产.

Optimization of Purification Process for Total Flavonoids from Glycyrrhiza uralensis by Macroporous Adsorption Resin

Objective: To optimize separation and purification technology of total flavonoids from Glycyrrhiza uralensis by macroporous adsorption resin. Method: Twelve different types of resin were selected with the amount of absorption and desorption ratio of total flavonoids as indexes,based on this,separation and purification technology of total flavonoids were optimized. Result: Purification effect of AB-8 type macroporous was the best, its optimum technology conditions were:the concentration of sample solution 0.2 g·mL^-1, maximum sample quantity 2 mL to 1 mL resin, velocity of absorption 0.5 BV·h^-1, pH of sample solution 6, 6 BV water to remove impurities at 1 BV·h^-1, eluted by 4 BV 70% ethanol at flow rate of 1 BV·h^-1.Under this optimized process,purity of total alkaloids in elution was 38%. Conclusion: This optimized process was rational, feasible and suitable for industrial production.