跑台运动对阿霉素联合醋酸戈舍瑞林诱导骨质疏松大鼠骨密度和骨代谢的影响

目的研究阿霉素(doxorubicin,DOX)和醋酸戈舍瑞林(goserelin acetate,GA)联合作用对大鼠骨密度和骨代谢的影响,以及跑台运动对DOX联合GA诱导的大鼠骨质疏松的防治效果。方法 8周龄雌性SD大鼠64只,被随机分为8组,每组8只:安静对照组(SED)、DOX干预组(SED+DOX)、GA干预组(SED+GA)、DOX和GA联合干预组(SED+DOX+GA)、跑台运动对照组(EX)、跑台运动结合DOX干预组(EX+DOX)、跑台运动结合GA干预组(EX+GA)、跑台运动结合DOX和GA联合干预组(EX+DOX+GA)。药物和跑台运动干预周期均为8周,8周后测试所有大鼠左侧股骨骨密度和血清骨代谢指标。结果与其相对应的非药物干预各组相比较,药物干预各组大鼠骨密度显著降低、骨形成指标ALP和BGP显著降低而骨吸收指标Ca2+和TRACP5b显著升高;与DOX单独干预组及GA单独干预组相比较,DOX和GA联合干预组大鼠骨密度显著降低、骨形成指标ALP和BGP显著降低而骨吸收指标Ca2+和TRACP5b显著升高;与其相对应的安静组相比较,跑台运动各组大鼠骨密度显著升高、骨形成指标ALP和BGP显著升高而骨吸收指标Ca2+和TRACP5b显著降低。结论 DOX和GA单独或联合作用均可导致大鼠骨质疏松症的发生,且DOX和GA联合作用诱导大鼠骨质疏松的程度显著大于DOX或GA单独作用的结果;跑台运动可以有效降低DOX和GA单独或联合作用诱导的大鼠骨质疏松。

The effect of treadmill exercise on bone mineral density and bone metabolism in doxorubicin combined with goserelin acetate-induced osteoporosis rats

Objective To explore the effects of doxorubicin (DOX) combined with goserelin acetate (GA) on bone mineral density and bone metabolism of rats, as well as the prevention effect of treadmill exercise on doxorubicin and goserelin acetate-induced osteoporosis. Methods Sixty-four 8-week-old male SD rats were randomly divided into 8 groups: sedentary control group (SED), DOX intervention group (SED+DOX), GA intervention group (SED+GA), DOX combined GA intervention group (SED+DOX+GA), treadmill exercise control group (EX), treadmill exercise and DOX intervention group (EX+DOX), treadmill exercise and GA intervention group (EX+GA), and treadmill exercise and DOX combined GA intervention group (EX+DOX+GA). Drug and treadmill exercise intervention period was 8 weeks. After 8 weeks, BMD of the left femur and serum bone metabolic markers of rats were tested. Results Compared to their corresponding non-pharmacological intervention groups, BMD and the bone formation indicators BGP and ALP in the DOX and GA intervention groups decreased significantly, and the bone resorption indicators Ca2+ and TRACP5b in the DOX and GA intervention groups increased significantly. Compared to DOX or GA intervention alone, combined DOX and GA intervention significantly decreased BMD and bone formation indicators ALP and BGP, and increased the bone resorption indicators Ca2+ and TRACP5b of the rats. Compared to their corresponding sedentary groups, BMD and the bone formation indicators BGP and ALP of the treadmill exercise groups increased significantly, and the bone resorption indicators Ca2+ and TRACP5b of the treadmill exercise groups decreased significantly. Conclusion DOX and GA alone or combination can lead to osteoporosis in rats, and the extent of osteoporosis was significantly greater in the combined DOX and GA-induced rats than in rat of DOX or GA used alone. Treadmill exercise can effectively reduce DOX and GA alone or their combination-induced osteoporosis.