沉默Caveolin-1基因对膀胱癌细胞增殖和凋亡的影响

目的探讨沉默 Caveolin-1（Cav-1）基因对人膀胱癌细胞株5637增殖和凋亡的影响。方法通过siRNA转染人膀胱癌细胞株5637（沉默组）,以转染 FAM-siRNA为阴性对照组,未转染细胞为正常对照组。Re-al-time PCR法检测Cav-1基因沉默的效率,CCK-8法检测各组细胞的增殖情况,流式细胞术检测各种细胞的凋亡情况。结果 siRNA转染可显著降低人膀胱癌细胞株5637中 Cav-1 mRNA 的表达水平。沉默组细胞的增强能力明显减低,而细胞凋亡率明显升高（均P＜ 0.05）。结论沉默Cav-1基因可抑制人膀胱癌细胞株5637的细胞增殖并促进细胞凋亡。

Effect of the Silencing of Caveolin-1 Gene on Proliferation and Apoptosis of Human Bladder Cancer Cells

Objective]To explore the effect of the silencing of caveolin-1（Cav-1）gene on the proliferation and apoptosis of human bladder cancer cell line 5637 in vitro.[Methods]Cav-1 gene was transfected into human blad-der cancer cell line 5637 by siRNA（silence group）.Negative control group was transfected with FAM-siRNA, while normal control group was not transfected with FAM-siRNA.Real-time PCR was used to detect the efficacy of the silencing of CAV-1 gene.CCK-8 method was used to detect the cell proliferation in each group.Cell apopto-sis was measured by flow cytometry.[Results]SiRNA transfection could significantly reduce the mRNA expres-sion of Cav-1 in human bladder cancer cell line 5637.In the silence group,cell proliferation was obviously de-creased and cell apoptosis was obviously increased（all P〈 0.05）.[Conclusion]The silencing of Cav-1 gene can in-hibit the proliferation and promote the apoptosis of human bladder cancer cells.