热休克蛋白70肽段抑制胶原诱导关节炎小鼠Th17细胞生成

目的:研究HSP70肽段对CIA小鼠Th17细胞的影响,探讨其抑制CIA小鼠炎症损伤的机制。方法:将DBA/1小鼠随机分为HSP70肽段实验组、阴性对照组、结核菌素(PPD)对照组、阳性对照组。除阴性对照组外,其它各组小鼠用牛Ⅱ型胶原蛋白诱导关节炎。通过关节评分和病理改变评价病变程度。取外周淋巴结用流式细胞仪检测Th17细胞的数量,用实时定量PCR检测Th17细胞相关调控因子。结果:注射HSP70肽段的CIA小鼠其Th17细胞数量明显低于阳性对照组和PPD对照组,促进Th17细胞分化发育的相关因子IL-6、IL-23、TGF-β1、RORγt的表达水平低于阳性对照组和PPD对照组(P<0.05)。结论:HSP70肽段可调节Th17细胞分化的调控因子,抑制Th17细胞的分化,进而减轻CIA小鼠的炎症程度。

Heat shock protein 70 peptide inhibits Th17 cells in collagen-induced arthritis mice

AIM: The effect of heat shock protein 70(HSP70) peptide on Th17 cells was studied in collagen-induced arthritis(CIA) mice model and the mechanism of its inhibitory effect on inflammation was also investigated.METHODS: DBA/1 mice were randomly divided into four groups including HSP70 peptide group,negative control group,PPD treated group and positive control group.Arthritis was induced by immunizing bovine typeⅡcollage,except negative control group.Pathology was evaluated by paw swelling and histological changes.The number of Th17 cells in peripheral lymph nodes was assayed by flow cytometry,and Th17 cell-associated regulatory factors were measured by real-time quantitative PCR.RESULTS:The number of Th17 cells in HSP70 peptide treated group was significantly lower than that of positive control group and PPD treated group.Expression levels of IL-6,IL-23,TGF-β1 and orphan nuclear receptor(RORγt) in HSP70 peptide group were markedly reduced than those of positive group and PPD group.CONCLUSION: HSP70 peptide could play a protective role on CIA mice through decreasing Th17 cell regulating factors and inhibiting the differentiation of Th17 cells.