| LC-MS/MS测定大鼠血浆中乌头碱的浓度 |
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| 引用本文： | 王志琪,景贤,曾嵘,陈尧,谭志荣. LC-MS/MS测定大鼠血浆中乌头碱的浓度[J]. 中国临床药理学与治疗学, 2012, 17(4): 371-375 |
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| 作者姓名： | 王志琪 景贤 曾嵘 陈尧 谭志荣 |
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| 作者单位： | 1. 湖南中医药大学药学院,长沙410208,湖南;湖南中医药大学干细胞中药调控与应用实验室,长沙4102083,湖南
2. 中南大学临床药理研究所,长沙410078,湖南 |
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| 基金项目： | 湖南省教育厅科学研究项目(08B056);国家自然科学基金项目(81001471);湖南省重点学科建设项目资助(1007) |
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| 摘 要： | 目的:建立LC-MS/MS法检测大鼠血浆中乌头碱的浓度。方法:以维拉帕米作内标,血浆样品经MTBE液液萃取后,采用HyPURITYCyano(150mm×2.1mm,5μm)柱分离,流动相为乙腈:10mmol/L甲酸铵=(70∶30,V/V),流速为0.30mL/min。然后采用电喷雾离子源(ESI源)正离子多反应监测(MRM)扫描分析,乌头碱和维拉帕米的离子选择通道分别为:m/z646.4→586.4和455.2→164.9。结果:乌头碱的线性范围为9.3～2390pg/mL,最低检测浓度为9.3pg/mL,日内和日间变异均<15%。结论:本方法灵敏度高,适用于乌头碱在鼠内的药物代谢动力学研究。
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| 关 键 词： | LC-MS/MS 乌头碱 药代动力学 |
Determination of aconitine concentration in rats plasma by LC-MS/MS |
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| WANG Zhi-qi , JING Xian , ZENG Rong , CHEN Yao , TAN Zhi-rong. Determination of aconitine concentration in rats plasma by LC-MS/MS[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(4): 371-375 |
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| Authors: | WANG Zhi-qi JING Xian ZENG Rong CHEN Yao TAN Zhi-rong |
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| Affiliation: | 1 School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,Hunan,China;2 Division of Stem Cell Regulation and Applicaton,Hunan University of Chinese Medicine,Changsha 410208,Hunan,China;3 Institute of Clinical Pharmacology,Central South University,Changsha 410078,Hunan,China |
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| Abstract: | AIM: To establish an LC-MS/MS method for determining the concentration of aconitine in rats plasma.METHODS: Verapamil was used as internal standard,the plasma samples extracted by MTBE were separated on a HyPURITY Cyano column(150 mm × 2.1 mm,5 μm) with mobile phase contained acetonitrile and 10 mmol/L ammonium formate(70∶30,V/V) at flow rate of 0.30 mL/min.The electrospray ionization(ESI source) in positive ion multiple reaction monitoring(MRM) were used for scanning analysis,then,aconitine and verapamil ion selective channels were: m/z 646.4→586.4 and 455.2→164.9,respectively.RESULTS:The linear range for aconitine was 9.3-2390 pg/mL.The lower limit of quantitation was 9.3 pg/mL,and the relative standard deviation of intra-day and inter-day were less than 15%.CONCLUSION: The established method is suitable for pharmacokinetic study of aconitine. |
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| Keywords: | LC-MS/MS Aconitine Pharmacokinetics |
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