低聚葡萄籽原花青素对异丙肾上腺素诱导心肌细胞肥大的影响

目的:在原代培养的新生大鼠心肌细胞上观察低聚葡萄籽原花青素(oligomeric grape seed proanthocyanidins,GSP)对异丙肾上腺素(isoproterenol,Iso)诱导心肌细胞肥大的影响。方法:采用SD大鼠乳鼠原代心肌细胞培养法,BCA法测定心肌细胞总蛋白含量;流式细胞仪检测心肌细胞内氧自由基(reactive oxygen species,ROS)含量;比色法检测丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果:1×10-5 mol/L Iso能明显诱导心肌细胞肥大,GSP可剂量依赖性地抑制Iso诱导心肌细胞肥大,抑制细胞内ROS的生成,降低MDA含量、提高SOD活性。结论:GSP对Iso诱导的心肌细胞肥大具有明显的逆转作用,其机制至少部分与抑制ROS生成、降低氧化应激水平有关。

Effect of oligomeric grape seed proanthocyanidins on prevention isoproterenol induced cardiomyocytes hypertrophy

AIM:To investigate the effect of oligomeric grape seed proanthocyanidins(GSP) on neonatal cardiomyocytes hypertrophy induced by isoproterenol(Iso).METHODS: Hypertrophy in cultured neonatal rat cardiac myocytes was induced by Iso.Total protein content was measured by BCA method;reactive oxygen species(ROS) were detected by flow cytometry;colorimetric method was employed to measured MDA content and SOD antivity.RESULTS:1×10-5 mol/L Iso can activate β-AR completely and induce cardiomyocytes hypertrophy markedly.GSP therapy potently inhibited the increase of protein content,ROS generation and MDA content,inhibited the decrease of SOD activity with a dose-dependent manner.CONCLUSION: GSP possess protective effect against Iso induced cardiomyocytes hypertrophy,this may be related to reducing the ROS generation and oxidative stress level,at least in part.