CTLA-4基因外显子1的49位点A／G多态性与吉兰-巴林综合征的关联研究

目的探讨细胞毒性T淋巴细胞抗原4（CTLA-4）基因外显子1的49位点A／G多态性与吉兰-巴林综合征（GBS）易患率的相关性。方法采用多聚酶链反应限制性片段长度多态性（PCR—RVt,P）技术分析10例吉兰-巴林综舍征患者及20例正常人CTLA-4基因外显子1的49位点A／G多态性。结果CTLA-4基因的3种基因型在GBS患者组中分布情况为：从型30．0％；AG型50．0％；GG型20．0％。在对照组中分布情况为：从型35．0％；AG型50．0％；GG型15．0％（P〉0．05）。CTLA-4／G49等位基因频率在GBS患者组为45．0％，在对照组为40％（P〉0．05）。结论CTLA-4基因外显子1的49位点A／G多态性与GBS无显著性关联。

The Association Between the CTLA-4 Exon 1 Polymorphism and Guillain-Barre Syndrome

Objective To investigate the association of cytotoxic Tlymphocyte associated antigen 4(CTLA-4) gene A/G polymorphism with susceptibility to Guillain-Barre syndrome.Methods The A/G transition polymorphism at position 49 exon1(codon17) of the CTLA-4 gene was determined by polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP) method in 10 patients with Guillain-Barre syndrome as well as 20 control subjects.Results The genotype(AA、AG and GG) distribution of CTLA-4 gene A/G polymorphism was 30.0%,50.0% and 20.0% in patients with Guillain-Barre syndrome,and 35.0%,50.0% and 15.0% in controls(P>0.05).The allele frenquency of CTLA-4/G49 in patients with GBS was 45.0% in patients with Guillain-Barre syndrome and 40.0% in controls(P>0.05).Conclusion The polymorphism of CTLA-4 gene was not significantly associated with Guillain-Barre syndrome.