|
|||||
|
|
| 巫山淫羊藿离体胚培养的研究 | |
| 引用本文： | 周海琴,朱国胜,郭巧生,刘作易,周宁.巫山淫羊藿离体胚培养的研究[J].中国中药杂志,2012,37(14):2046-2051. |
| 作者姓名： | 周海琴 朱国胜 郭巧生 刘作易 周宁 |
| 作者单位： | 1. 南京农业大学中药材研究所,江苏南京,210095 2. 贵州省现代中药材研究所,贵州贵阳,550006 3. 贵州同济堂制药有限公司,贵州贵阳,550002 |
| 基金项目： | 国家科技支撑计划项目(2009BAI74B02);贵州省科技计划项目(黔科合院所创能[2010]4002);中央补助地方科技基础条件专项基金项目(黔科条中补地[2010]4002号) |
| 摘 要： | 目的:研究药用植物巫山淫羊藿组织培养技术,为工厂化育苗提供科学根据。方法:以巫山淫羊藿的种胚为外植体,采用MS培养基,附加不同浓度2,4-D,6-BA,IBA,NAA进行正交试验。结果:诱导愈伤组织的最优培养基为:MS+2,4-D2 mg.L-1+IBA 2 mg.L-1+NAA 0.5 mg.L-1;愈伤组织分化的最优培养基为:MS+6-BA 1 mg.L-1+NAA 0.5 mg.L-1+IBA 1 mg.L-1;诱导芽的最优培养基为:MS+IBA 2 mg.L-1+6-BA 0.5 mg.L-1;芽增殖的最佳培养基为:MS+6-BA 1.0 mg.L-1+NAA 0.5 mg.L-1。结论:通过诱导愈伤组织途径和丛生芽途径,建立了巫山淫羊藿种胚外植体诱导和培养方法,达到快速繁殖的目的。 |
| 关 键 词： | 巫山淫羊藿 种胚 组织培养 植株再生 |
| 收稿时间： | 2012/2/26 0:00:00 |
In vitro embryo culture of Epimedium wushanense |
|
| ZHOU Haiqin,ZHU Guosheng,GUO Qiaosheng,LIU Zuoyi and ZHOU Ning.In vitro embryo culture of Epimedium wushanense[J].China Journal of Chinese Materia Medica,2012,37(14):2046-2051. | |
| Authors: | ZHOU Haiqin ZHU Guosheng GUO Qiaosheng LIU Zuoyi and ZHOU Ning |
| Institution: | Institute of Chinese Medicinal, Nanjing Agricultural University, Nanjing 210095, China;Institute of Morden Chinese Medical Materials, Guizhou Academy of Agricaltural Sciences, Guiyang 550006, China;Institute of Chinese Medicinal, Nanjing Agricultural University, Nanjing 210095, China;Institute of Morden Chinese Medical Materials, Guizhou Academy of Agricaltural Sciences, Guiyang 550006, China;Guizhou Tongjitang Pharmaceutical Co., Ltd., Guiyang 550002, China |
| Abstract: | Objective: To study the in vitro embryo culture of Epimedium wushanense and provide scientific basis for large scale production of tissue culture. Method: Cullus and buds were induced from embryo of E. wushanense on a MS medium supplemented with different 2,4-D,6-BA,NAA,IBA. Result: The optimal compositions of medium that induced callus and buds from embryo were the MS medium supplemented with 2,4-D 2 mg·L-1,IBA 2 mg·L-1 and NAA 0.5 mg·L-1 and the MS medium supplemented with IBA 2 mg·L-1 and 6-BA 0.5 mg·L-1,respectively. The optimum medium for callus differentiation was MS+6-BA 1 mg·L-1+NAA 0.5 mg·L-1+IBA 1 mg·L-1, and MS+6-BA 1.0 mg·L-1 +NAA 0.5 mg·L-1 for shoots proliferation. Conclusion: Using embryo as explants, the method of induction and culture of E. wushanense was established by the callus and buds, and the embryo of E. wushanense can be quickly propagated. |
| Keywords: | Epimedium wushanense embryo tissue culture plant regeneration |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |