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| 硒化壳聚糖对NB4细胞血管内皮生长因子表达及分泌的影响 | |
| 引用本文： | 邓守恒,曹凤军,李芳,邓守平,陈萍. 硒化壳聚糖对NB4细胞血管内皮生长因子表达及分泌的影响[J]. 陕西医学杂志, 2010, 39(4): 387-389 |
| 作者姓名： | 邓守恒 曹凤军 李芳 邓守平 陈萍 |
| 作者单位： | 1. 郧阳医学院附属人民医院肿瘤中心,十堰,442000 2. 郧阳医学院附属人民医院消化内科 |
| 基金项目： | 湖北省教育厅资助项目，十堰市科学技术局基金 |
| 摘 要： | 目的:探讨硒化壳聚糖对白血病细胞株NB4细胞VEGF mRNA表达及VEGF蛋白分泌的变化。方法:应用RT-PCR法和ELISA法研究硒化壳聚糖对NB4细胞VEGF mRNA表达及VEGF蛋白分泌的影响。结果:NB4细胞中有VEGF mRNA的表达及VEGF蛋白的分泌,硒化壳聚糖在体外能下调VEGF mRNA的表达和VEGF蛋白的分泌,呈剂量依赖关系。结论:硒化壳聚糖除可通过诱导NB4细胞分化或诱导其凋亡外,还可通过抑制促血管新生达到发挥抗白血病的效应。 |
| 关 键 词： | 壳多糖 硒化合物 白血病,粒细胞,急性/免疫学 血管生成因子/分析 内皮,血管 @NB4 |
Effect of selenium chitosan on the expression and secretion of VEGF in NB4 cells |
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| Center of Oncology,RenMing Hospital,YunYang Medical collegeDeng Shouheng Cao Fengjun Li Fang et al. Effect of selenium chitosan on the expression and secretion of VEGF in NB4 cells[J]. Shaanxi Medical Journal, 2010, 39(4): 387-389 | |
| Authors: | Center of Oncology RenMing Hospital YunYang Medical collegeDeng Shouheng Cao Fengjun Li Fang et al |
| Affiliation: | Center of Oncology,RenMing Hospital,YunYang Medical college(Shiyan 442000)Deng Shouheng Cao Fengjun Li Fang et al |
| Abstract: | Objective:TO investigate the expression of VEGF mRNA and secretion of VEGF protein in NB4 cells treated by selenium chitosan . Methods:Semi-quantitative RT-PCR and ELISA were used to study the expression of VEGF mRNA and secretion of VEGF protein in NB4 cell treated by selenium chitosan . Results:VEGF was expressed in NB4 cell. The expression of VEGF mRNA and secretion of VEGF protein could be down regulated by selenium chitosan in a dose dependent manner. Conclusion:Besides inducing apoptosis and promoting... |
| Keywords: | @NB4 |
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