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高效液相色谱法测定红药胶囊中人参皂苷Rg1的含量
引用本文:王仿成,周文艳. 高效液相色谱法测定红药胶囊中人参皂苷Rg1的含量[J]. 安徽医药, 2008, 12(5): 424-425
作者姓名:王仿成  周文艳
作者单位:安徽省岳西县人民医院,安徽,岳西,246600;合肥康诚科技有限公司,安徽,合肥,230023
摘    要:
目的建立一种高效液相色谱法测定红药胶囊中人参皂苷Rg1的含量。方法以ODS-C18为固定相,乙腈-0.2%磷酸(20:80)为流动相,检测波长为203nm。结果人参皂苷Rg1线性范围为1.0-20μg,r=0.9998,加样回收率为98.9%,RSD为0.5%。结论该方法简便、可靠、准确,可用于红药胶囊的质量控制。

关 键 词:高效液相色谱法  红药胶囊  人参皂苷Rg1

Determination of ginsenoside Rg1 in hongyao capsules by HPLC
WANG Fang-cheng,ZHOU Wen-yan. Determination of ginsenoside Rg1 in hongyao capsules by HPLC[J]. Anhui Medical and Pharmaceutical Journal, 2008, 12(5): 424-425
Authors:WANG Fang-cheng  ZHOU Wen-yan
Affiliation:WANG Fang-cheng,ZHOU Wen-yan(1.The People' s Hospital of Yuexi Country, Yuexi 246600, China ; 2. Hefei Kangcheng Technology Ltd, Hefei 230023, China )
Abstract:
Aim To establish an HPLC method for determination of ginsenoside RgI in hongyao capsules. Methods In this study, ODS-C18 was adopted as stationary phase with the mobile phase of acetonitrile -0.2% phosphoric acid (20:80) , and the detection wavelength was at 203 nm. Result The linear range of ginsenoside Rg1 was 1.0 - 20 μg, r=0.9998. The average recovery was 98.9% , and RSD was 0.5%. Conclusion This method is simple, reliable,accurate and can be used as a quality control of hongyao capsules.
Keywords:HPLC  hongyao capsules  ginsenoside Rg1
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