Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system |
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| Authors: | Lin Gang Yu-chen Yao Ying-fu Liu Yi-peng Li Kai Yang Lei Lu Yuan-chi Cheng Xu-yi Chen Yue Tu |
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| Affiliation: | 1.Neurological Hospital of Affiliated Hospital of Logistics University of Chinese People''s Armed Police Force, Tianjin, China;2.Tianjin University of Traditional Chinese Medicine, Tianjin, China;3.Logistics University of Chinese People''s Armed Police Forces, Tianjin, China;4.Liaoning Medical University, Jinzhou, Liaoning Province, China;5.Key Laboratory of Nerve Trauma Repair, Tianjin, China |
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| Abstract: | ![]()
We present a novel in vitro model in which to investigate the efficacy of experimental drugs for the promotion of axon regeneration in the central nervous system. We co-cultured rat hippocampal neurons and cerebral cortical oligodendrocytes, and tested the co-culture system using a Nogo-66 receptor antagonist peptide (NEP1–40), which promotes axonal growth. Primary cultured oligodendrocytes suppressed axonal growth in the rat hippocampus, but NEP1–40 stimulated axonal growth in the co-culture system. Our results confirm the validity of the neuron-oligodendrocyte co-culture system as an assay for the evaluation of drugs for axon regeneration in the central nervous system. |
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| Keywords: | nerve regeneration experimental models NEP1–40 oligodendrocytes neurons axon regeneration Nogo PC12 cells neural regeneration |
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