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Geniposide prevents rotenone-induced apoptosis in primary cultured neurons
Authors:Lin Li  Juan Zhao  Ke Liu  Guang-lai Li  Yan-qing Han  Yue-ze Liu
Affiliation:1.Key Laboratory of Cellular Physiology, Shanxi Medical University, Taiyuan, Shanxi Province, China;2.Second Hospital, Shanxi Medical University, Taiyuan, Shanxi Province, China
Abstract:
Geniposide, a monomer extracted from gardenia and widely used in Chinese medicine, is a novel agonist at the glucagon-like peptide-1 receptor. This receptor is involved in neuroprotection. In the present study, we sought to identify an anti-apoptotic mechanism for the treatment of neurodegenerative diseases. Primary cultured neurons were treated with different concentrations of rotenone for 48 hours. Morphological observation, cell counting kit-8 assay, lactate dehydrogenase detection and western blot assay demonstrated that 0.5 n M rotenone increased lactate dehydrogenase release, decreased the expression of procaspase-3 and Bcl-2, and increased cleaved caspase-3 expression in normal neurons. All these effects were prevented by geniposide. Our results indicate that geniposide diminished rotenone-induced injury in primary neurons by suppressing apoptosis. This may be one of the molecular mechanisms underlying the efficacy of geniposide in the treatment of neurodegenerative diseases.
Keywords:nerve regeneration   geniposide   rotenone   cell apoptosis   Alzheimer''s disease   caspase-3   Bcl-2   neural regeneration
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