In vitro model of the blood-brain barrier established by co-culture of primary cerebral microvascular endothelial and astrocyte cells |
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| Authors: | Yan Wang Ning Wang Biao Cai Guang-yun Wang Jing Li Xing-xing Piao |
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| Affiliation: | Key Laboratory of Xin’an Medicine, Ministry of Education; Institute for Pharmacodynamics and Safety Evaluation of Chinese Medicine, Anhui Academy of Chinese; College of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui Province, China |
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| Abstract: | Drugs for the treatment and prevention of nervous system diseases must permeate the blood-brain barrier to take effect. In vitro models of the blood-brain barrier are therefore important in the investigation of drug permeation mechanisms. However, to date, no unified method has been described for establishing a blood-brain barrier model. Here, we modified an in vitro model of the blood-brain barrier by seeding brain microvascular endothelial cells and astrocytes from newborn rats on a polyester Transwell cell culture membrane with 0.4-µm pores, and conducted transepithelial electrical resistance measurements, leakage tests and assays for specific blood-brain barrier enzymes. We show that the permeability of our model is as low as that of the blood-brain barrier in vivo. Our model will be a valuable tool in the study of the mechanisms of action of neuroprotective drugs. |
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| Keywords: | nerve regeneration blood-brain barrier astrocytes brain microvascular endothelial cells permeability co-culture Transwell chamber neural regeneration |
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