uPA、uPAR在舌鳞癌中的表达及意义

目的:研究尿激酶型纤溶酶原激活剂(uPA)及其特异受体(uPAR)在舌鳞癌(TSCC)中的表达及其与临床病理参数的关系。方法:应用免疫组化SABC法检测uPA、uPAR在74例TSCC组织和15例癌旁正常黏膜中的表达。Spearman法和Mann-WhitneyU法分析其表达的相关性及与舌鳞癌临床病理参数的关系,χ2检验分析其与颈淋巴结转移的关系。结果:TSCC组织中uPA和uPAR的阳性表达率分别为71.6%和64.8%,且表达呈正相关(P<0.05)。uPA的表达水平与临床分期、颈淋巴结转移相关,与肿瘤病理学分级无关;uPAR与颈淋巴结转移相关,与临床分期、肿瘤病理学分级无关。uPA和uPAR同时阳性表达组,颈淋巴结转移率显著高于同时阴性表达组,两组间比较,差异有非常显著意义(P<0.05)。结论:uPA和uPAR在TSCC中明显高表达,并与TSCC的颈淋巴结转移密切相关。

Expressions and clinical significance of urokinase-type plasminogen activator (uPA) and uPA receptor (uPAR) in tongue squamous cell carcinoma

PURPOSE:To study the expressions and clinical significance of urokinase-type plasminogen activator(uPA)and uPA receptor(uPAR)in tongue squamous cell carcinoma(TSCC).METHODS:The expressions of uPA and uPAR at protein level were examined in 74 cases of TSCC and 15 cases of normal peripheral tissues around cancer(as control)by strep avidin-biotin complex(SABC)immunohistochemical technique.The relationship between the expressions of uPA and uPAR was evaluated by the Spearman's rank correlation test.Mann-Whitney U-test was used to examine the association of these factors with conventional clinical pathological factors.Pearson's Chi-square test was used to analyze the relationship between uPA,uPAR and cervical lymph node status.RESULTS:The rates of the positive expression in TSCC of uPA and uPAR were 71.6% and 64.8% respectively,and a positive relationship between expression of uPA and uPAR was found(P<0.05).There was significant correlation between the level of uPA expression and TNM stage or cervical lymph node status,either between the level of uPAR expression and cervical lymph node status.Pathological grade was not significantly related to the level of uPA expression.Pathological grade and TNM stage were not significantly related to the level of uPAR expression.TSCC cases with concurrent positive expression of these proteins revealed significant higher tendency of cervical lymph node metastasis than those with concourse negative expression(P<0.05).CONCLUSIONS:The expression of uPA and uPAR increased in TSCC,and uPA,uPAR may contribute significantly to TSCC invasion and metastasis.