溃疡性结肠炎大鼠细胞因子及T细胞亚群变化的实验研究

目的研究UC大鼠的免疫功能的变化，包括细胞因子及T细胞亚群的变化。方法以TNBS／L醇方法制备UC大鼠模型，检测UC大鼠细胞因子TNF-α、IL-2、IL-4及T细胞亚群的变化。结果模型组血清TNF-α、IL-2含量及外周血CD3^＋、CD4^＋细胞百分比在1、3,7周3个时间段均升高（P〈0．05）。而血清IL-4含量在1、7周时UC大鼠与对照组比较差异无统计学意义（P〉0．05），3周时出现降低，与其它组比较差异有统计学意义（P〈0．05）；CO3^＋细胞百分比较正常组降低（P〈0．05）；CD；／CD8^＋比值明显高于正常组（P〈0．05）。结论 T细胞亚群及细胞因子的变化，说明UC大鼠存在的细胞免疫功能亢进，进而引发肠粘膜局部的变态反应。

The experiment study on the cytokine and the T cell subset of rat ulcerative colitis

Objective To investigate the variation of immune function in UC rats, including T cell subset, the cytoldne of TNF-α, IL-2,IL-4.Methods To bulid UC animal model by TNBS/alcohol,ELISA was used to clctcct the cytokine of TNF-α,IL-2,IL-4.Flow cytometry was used to detect the T cell subset. Results The content of TNF-α,IL-2 in blood serum was predominantly increased at the first, third and seven week in model group than in normal group （ P 〈 0.05 ）. The content of IL-4 in blood serum hadn＇ t significant deviation at the first,seven week between model group and normal group （ P 〉 0.05） .The percentage of CD3^＋ ,C, D4^＋ in pefiphefial blood was predominantly increased at the first,third and seven week in model group than in normal group（ P 〈 0.05） ,but the percentage of CIX＋ was lower than normal group（ P 〈 0.05 ）, the ratio of CD4^＋/CD8^＋ was higher than normal group （ P 〈 0.05 ）. Conclusion The results show the abnormal increasing of cell immunoresponse,it may cause the intestinal mucous activate the local allergy.