神经生长因子对老年鼠穹窿海马伞损伤后齿状回胆碱能突触重构的作用

损伤老年鼠左侧穹窿海马伞，造成隔-海马胆碱能系统损害模型。用免疫电镜和形态计量学分析NGF对齿状回分子层受体（NGFr）阳性突触和胆碱乙酰转移酶（ChAT）阳性轴突终未的影响。结果显示：损伤一个月后，损伤侧齿状回分子层NGFr阳性突触前终末、实触后致密物和ChAT阳性轴突终末面积和周长都有不同程度的缩小；脑室注射NGF，损伤侧齿状回分子层NGFr阳性突触和ChAT阳性实触终末都有一定的恢复．提示NGF对齿状回分子层受损的NGFr阳性突触和ChAT阳性轴突终未的重构具有促进作用。

EFFECT OF NERVE GROWTH FACTOR ON CHOLINERGIC SYNAPTIC REMODELLING OF DENTATE GYRUS IN AGED RATS WITH FIMBRIA FORNIX TRANSECTION

In this study EM quantitative analysis were used to examine whether exogenous nerve growth factor(NGF) could affect the cholinergic terminals and NGF receptor (NGFr)-positive synaptic connections in dentate gyrus in aged rats with fimbria fornix transection. Aged rats received NGF or vehicle intracerebroventricularly twice a week for 30 days after lesion.Rats received vehicle showed morphological change of cholinergic terminals and NGFr-positive elements, the average area and perimeter of cholinergic terminals and NGFr-positive presynaptic membrane, postsynaptic dense structure and cholinergic terminals were all significantly decreased (P<0. 01 ). In the rats received exogenous NGF, the average area and perirneter of oholinergic terminals and NGFr-positive synaptic eIements mentioned above were recovered to about normal levels,The results suggested that exogenous NGF could cause reorganization of cholinergic tertninals and NGFr-positive synaptic elements in the aged rats with fimbria fornix transection.