反相高效液相色谱法测定(E)-3-(4-((3,5,6-三甲基吡嗪-2-基)甲氧基)-3-甲氧基苯基)丙烯酸在SD大鼠血浆中的浓度

目的 建立测定SD大鼠血浆中(E)-3-(4-((3,5,6-三甲基吡嗪-2-基)甲氧基)-3-甲氧基苯基)丙烯酸浓度的反相高效液相色谱(LC-UV)方法,并用于其在SD大鼠体内的药动学研究。方法 采用Diamonsil-C₁₈(2)柱(100 mm×4.6 mm,5 μm),以乙腈:水(含5 mM的醋酸铵,用醋酸调节pH至4.0)=30:70为流动相,测定70只SD大鼠单剂量尾静脉给药15 mg/kg后不同时刻血浆中药物的浓度,并由此计算其在SD大鼠体内的药动学参数。结果 SD大鼠

Determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate in rat plasma by RP-HPLC

Objective To establish a method for determination of(E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate in plasma of SD rats by RP-HPLC.Methods The chromatographic separation was performed on a Diamonsil-C18 column(100 mm×4.6 mm,5 μm) with the mobile phase consisting of acetonitrile/5 mM ammonium acetate buffer(pH 4.0)(30/70,v/v).The single intravenous injection of(E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate was given to 70 SD rats to study the pharmacokinetics.Results The intra-run and inter-run RSD were less than 15%.The pharmacokinetics parameters were as follows: Cmax was 35.60 μg/ml,t1/2 was 3.36 h,AUC0～12 was 17.12 μg h/ml,AUC0～∞ was 17.72μg h/ml.Conclusion The method was simple and accurate,which could be used to separation and determination of(E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate.