| RP-HPLC制备色谱法分离淡豆豉中高纯度异黄酮苷元 |
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| 引用本文： | 曲丽萍,宓鹤鸣,范国荣.RP-HPLC制备色谱法分离淡豆豉中高纯度异黄酮苷元[J].中药材,2005,28(12):1062-1063. |
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| 作者姓名： | 曲丽萍 宓鹤鸣 范国荣 |
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| 作者单位： | 第二军医大学药学院药物分析教研室,上海,200433 |
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| 摘 要： | 淡豆豉经大孔树脂柱色谱初步分离纯化,采用RP-HPLC制备色谱法,乙腈-水-冰醋酸(35∶ 65∶ 1,v/v/v)为流动相,流速3.0 ml/min,检测波长260 nm.结果表明,在30 min内快速分离到大豆素、大豆黄素和染料木素,产物纯度均达到99%以上.
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| 关 键 词： | 高效液相制备色谱 大孔树脂 淡豆豉 异黄酮苷元 |
| 收稿时间： | 2005-09-26 |
| 修稿时间： | 2005年9月26日 |
Preparation of Soybean Isoflavone Aglycones by Reversed-Phase High Performance Liquid Chromatography from Semen Sojae Praeparatum |
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| Qu Liping,Mi Heming,Fan Guorong.Preparation of Soybean Isoflavone Aglycones by Reversed-Phase High Performance Liquid Chromatography from Semen Sojae Praeparatum[J].Jorunal of Chinese Medicinal Materials,2005,28(12):1062-1063. |
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| Authors: | Qu Liping Mi Heming Fan Guorong |
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| Institution: | Department of Pharmaceutical Analysis, College of Pharmacy, Second Military Medical University, Shanghai 200433 |
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| Abstract: | Objective:An efficient method for the isolation of isoflavone aglycones from Semen Sojae Praeparatum was developed.Methods:The crude isoflavones were extracted by 75% aqueous ethanol and primarily purified by 1300 macroporous resin,and then the fractions containing daidzein,glycitein and genistein were obtained and subjected to preparative HPLC for the isolation of target components.Results:Daidzein,glycitein and genistein were obtained with purities of over 99%,determined by analytical HPLC.Conclusion:This technique is simple and suitable for the isolation of isoflavone aglycones from Semen Sojae Praeparatum. |
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| Keywords: | Preparative HPLC Macroporous resin Semen Sojae Praeparatum Soflavone aglycones |
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