LS8 cell apoptosis induced by NaF through p-ERK and p-JNK – a mechanism study of dental fluorosis |
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| Authors: | Lin Zhao Juedan Li Jiali Su Malcolm L Snead |
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| Institution: | 1. Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, Department of Preventive Dentistry, Stomatology Hospital, Xi'an Jiaotong University, Xi’an, PR China;2. Department of Oral Pathology, Stomatology Hospital, Ningxia Medical University, Yinchuan, PR China;3. Department of Endodontics, Yinchuan Stomatology Hospital, Yinchuan, PR China;4. Herman Ostrow School of Dentistry of USC, Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA, USA |
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| Abstract: | Objective: To investigate the possible biological mechanism of dental fluorosis at a molecular level.Material and methods: Cultured LS8 were incubated with serum-free medium containing selected concentrations of NaF (0?～?2?mM) for either 24 or 48?h. Subcellular microanatomy was characterized using TEM; meanwhile, selected biomolecules were analysed using various biochemistry techniques. Transient transfection was used to modulate a molecular pathway for apoptosis.Results: Apoptosis of LS8 was induced by NaF treatment that showed both time and concentration dependency. The activity of caspase-3, -8, -9 was found to be increased with NaF in a dose-dependent manner. Western blot revealed that the protein expression of p-ERK and p-JNK were decreased, while the expression of p-P38 was increased. Inhibition of the p-ERK and p-JNK pathways resulted in a similar decrease for caspase-3.Conclusion: During NaF-induced apoptosis of LS8, p-ERK and p-JNK were closely associated with induction of apoptosis, which might be a mechanism of dental fluorosis. |
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| Keywords: | NaF LS8 apoptosis mitogen-activated protein kinase dental fluorosis |
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