| 脂质体介导p27基因转染对喉癌细胞系Hep-2生长影响的研究 |
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| 引用本文： | 孙安,孙永柱,高卓平,席爱民,段学军,陈谊辉. 脂质体介导p27基因转染对喉癌细胞系Hep-2生长影响的研究[J]. 中国耳鼻咽喉头颈外科, 2009, 0(11): 632-635 |
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| 作者姓名： | 孙安 孙永柱 高卓平 席爱民 段学军 陈谊辉 |
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| 作者单位： | 西安市第四医院耳鼻咽喉科;第四军医大学唐都医院耳鼻咽喉科; |
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| 基金项目： | 陕西省自然科学基金资助项目(SJ08-ZT09) |
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| 摘 要： | 目的探讨p27基因对喉癌Hep-2细胞系生长的抑制作用,为喉癌发生机制的研究和基因治疗提供理论依据。方法采用基因转染技术,将p27cDNA转染到喉癌Hep-2细胞系中,了解其对细胞增殖能力及细胞周期的影响。结果转染p27基因的喉癌细胞生长受到抑制,其在软琼脂上克隆形成能力降低了大约4倍。对细胞周期分析表明,处在G0～G1期的细胞由32.2%增加到66.9%,经Dotblot、Western blot杂交和免疫组化证实,p27mRNA表达有明显差异（P〈0.01）,p27的蛋白表达量有明显差异（P〈0.01）,说明p27蛋白具有生长抑制功能,其作用点为G1～S期。结论提高喉癌细胞中p27的表达,能抑制肿瘤细胞的生长,导入p27基因是一种治疗喉癌的新途径。
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| 关 键 词： | 喉肿瘤 基因 肿瘤抑制 基因表达 转染 |
Effects of exogenous p27 gene on the cell cycle and proliferation of Hep-2 laryngeal cancer cell line |
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| SUN An,SUN Yongzhu,GAO Zhuoping,XI Aimin,DUAN Xuejun,CHEN Yihui. Effects of exogenous p27 gene on the cell cycle and proliferation of Hep-2 laryngeal cancer cell line[J]. Chinese Archives of Otolaryngology-Head and Neck Surgery, 2009, 0(11): 632-635 |
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| Authors: | SUN An SUN Yongzhu GAO Zhuoping XI Aimin DUAN Xuejun CHEN Yihui |
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| Affiliation: | SUN An1,SUN Yongzhu2,GAO Zhuoping1,XI Aimin1,DUAN Xuejun1,CHEN Yihui1 (1 Department of Otolaryngology,the Forth Hospital of Xi'an,Xi'an,Shanxi,710004,China,2 Department of Otorhinolaryngology,Tangdu Hospital,Fourth Military Medical University,710038,China) |
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| Abstract: | OBJECTIVE To explore the effect of p27 gene on the growth inhibition of laryngeal carcinoma cell line Hep-2. METHODS The p27 cDNA was transfected into human laryngeal carcinoma cell line(Hep-2 cells)with lipofectamine. The growth rate, cell cycles and colony formation rate of transfected cells were observed by means of MTT and FCM assay. RESULTS Expression of p27 in Hep-2 was identified by Dot blot, immunohistochemical method and Western blot analysis. The growth rate of Hep-2 transfected with p27 gene was ... |
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| Keywords: | Laryngeal Neoplasms Gnes Tumor Suppressor Gene Expression Transfection |
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