重组骨脱细胞细外基复合Wistar大鼠成骨细胞体外培养的形态学观察

目的新型重组骨脱细胞细胞外基质(REAECM)的制备及其细胞相容性的初步检测，为骨组织工程寻找一种新型的细胞外支架提供实验依据。方法应用体外细胞培养技术，对鼠成骨细胞和REAECM体外进行联合培养1-4周，通过相差显微镜、光镜、电镜观察细胞在材料中的生长情况。结果成骨细胞可以在REAECM上发生良好的黏附、增殖，并且可以长入REAECM的孔隙内。结论REAECM可作为构建组织工程骨的一种较好的支架材料，具有网状孔隙结构；在体外和成骨细胞复合培养时表现出良好的细胞相容性，可以作为一种天然的骨组织替代材料。

THE MORPHOLOGICAL OBSERVATION OF THE OSTEOBLAST CULTURED WITH RECONSTRUCTED ACELLULAR EXTRACELLULAR MATRIX IN VITRO

Objective The construction of the reconstructed acellular extracellular matrix(REAECM) of bone and preliminary investigation of biocompatibility of REAECM was performed to supply the experimental basis for the extracellular scaffold of bone tissue engineering. Methods Applying the technique of culture in vitro, biocompatibility of the REAECM cultured with osteoblasts was observed using phase-contrast microscope and scanning eletron microscope at 1-4 weeks. Results The osteoblasts could attach and proliferate, even grow well into the foam of REAECM.Conclusion REAECM is an excellent scaffold material for bony tissue engineering construction, which pocesses a morphological structure of three-dimensional foam. The REAECM showed a good biocompatibility with osteoblasts co-cultured in vitro. There was prospective signs of REAECM to the attachment, proliferation of the osteoblasts