卡维地洛对多柔比星诱导心肌病大鼠氧化应激的影响

目的:探讨卡维地洛(carvedilol,CVD)对多柔比星(adriamycin,ADR)致心肌病大鼠氧化应激水平的影响及其可能机制。方法:采用腹腔注射多柔比星建立心肌病大鼠模型,随机分为卡维地洛组和多柔比星组,每组10只。另取正常大鼠10只为正常对照组。卡维地洛组每日给予卡维地洛60 mg/kg;多柔比星组、正常对照组每日给予相同体积生理盐水,观察8周。第9周行心脏超声和血流动力学检测,测定心室胶原含量,总抗氧化能力和丙二醛浓度。结果:卡维地洛组与多柔比星组相比,可显著提高左室射血分数(P<0.01);降低心肌胶原容积分数(P<0.01)。与正常对照组相比,多柔比星组总抗氧化能力显著降低(P<0.01),丙二醛浓度显著升高(P<0.01);而与多柔比星组相比,卡维地洛组可显著提升总抗氧化能力(P<0.01),明显降低丙二醛浓度(P<0.05)。结论:卡维地洛能显著改善多柔比星引起的血流动力异常,改善心脏收缩与舒张功能,逆转心肌重塑,可能与其提高心肌抗氧化应激能力有关。

Influence of carvedilol on oxidative stress in rat with adriamycin-induced cardiomyopathy

Objective: To investigate the influence of oxygen stress in rat with adriamycin-induced cardiomyopathy,and to intervene with the third-generation blocker: carvedilol to explore its possible mechanism.Methods: Male Sprague-Dawley rats were administered adriamycin intraperitoneally injected.Randomly divided into two groups(n=10 in each group): carvedilol group(60 mg/kg daily),adriamycin group and another normal heart control group(giving an equivalent volume of 0.9% saline daily).After 8 weeks of remedy,echocardiography,hemodynamic parameters,the volumetric fraction of collagenous fibre(CVF) and oxidative stress including total antioxidation capacity(T-AOC) and malondialdehyde(MDA) were studied. Results: Compared with adriamycin group,carvedilol group can significantly improve left ventricular ejection fraction(P<0.01) and depress CVF obviously(P<0.01).Compared with control group,adriamycin group can significantly decrease the activity of T-AOC(P<0.01) and improve the contents of MDA greatly(P<0.01).The activity of T-AOC in the carvedilol group was enhanced significantly compared with that in the adriamycin group(P<0.01).The contents of MDA in the carvedilol group were decreased compared with that in the adriamycin group(P<0.05). Conclusion: Carvedilol can relieve adriamycin-induced hemodynamic abnormality evidently,improve cardiac systolic and diastolic function greatly and reverse ventricular remodeling.This mechanism may be concerned with its improvement to oxygen stress.