| 高效液相色谱法测定小儿润肺止咳口服液中橙皮苷的含量 |
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| 引用本文： | 姜韧,刘刚,宋婧,李勉珊,王海涛,赵淼,谭生建.高效液相色谱法测定小儿润肺止咳口服液中橙皮苷的含量[J].解放军药学学报,2010,26(5):438-439. |
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| 作者姓名： | 姜韧 刘刚 宋婧 李勉珊 王海涛 赵淼 谭生建 |
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| 作者单位： | 1. 解放军306医院药学部,北京,100101
2. 沧州医学高等专科学校药学系,河北沧州,061000 |
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| 摘 要： | 目的建立高效液相色谱法测定小儿润肺止咳口服液中橙皮苷含量的方法。方法色谱柱用十八烷基硅烷键合硅胶为固体相（150mm×4.6mm,5μm）;流动相：A为10%甲醇-甲醇（75：25）,流速：1ml/min,检测波长：284nm。结果以峰面积对进样浓度线性回归,得回归方程：Y=0.05803X-1.242,线性范围：25.20～126.0μg/ml,r=0.9999。橙皮苷回收率为99.5%;RSD为2.0%。结论本法测定结果准确,操作简便,精密度好,可用于小儿润肺止咳口服液中橙皮苷的含量测定。
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| 关 键 词： | 高效液相色谱法 小儿润肺止咳口服液 橙皮苷 |
Determination of Hesperidin In Xiao'er Runfeizhike Koufuye by High Performance Liquid Chromatography |
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| JIANG Ren,LIU Gang,SONG Jing,LI Mian-shan,WANG Hai-tao,ZHAO Miao,TAN Sheng-jian.Determination of Hesperidin In Xiao'er Runfeizhike Koufuye by High Performance Liquid Chromatography[J].Pharmaceutical Journal of Chinese People's Liberation Army,2010,26(5):438-439. |
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| Authors: | JIANG Ren LIU Gang SONG Jing LI Mian-shan WANG Hai-tao ZHAO Miao TAN Sheng-jian |
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| Institution: | (①Department of Pharmacy,Hospital 306 of PLA,Beijing100101,China;②Department of Pharamacy,Medical College of Cangzhou,Cangzhou061000,China ) |
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| Abstract: | Objective To develop an high performance liquid chromatography quantitative method for determination of hesperidin in Xiao＇er Runfeizhike Koufuye.Methods The chromatographic conditions included column C18（150 mm ×4.6 mm,5 μm）and the mobile phase of 10% methanol-methanol（75：25）.The flow rate was 1 ml/min and monitored at 284 nm.Results The regress equation for hesperidin was Y=0.0580 3X-1.242,r=0.9999 and the linear range was 25.20-126.0 μg/ml.The average recovery of hesperidin was 99.5%,RSD 2.0%.Conclusion This method is simple,time-saving and accurate.It can be used for routine analysis of hesperidin in Xiao＇er Runfeizhike Koufuye. |
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| Keywords: | high performance liquid chromatography Xiao＇er Runfeizhike Koufuye hesperidin |
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