国际各类品种血小板膜糖蛋白单克隆抗体试剂对血小板抗原单克隆抗体特异性免疫固定检测技术的影响

本研究按国际输血学会第14届国际血小板免疫学研讨会和合作研究项目的要求，分析比较国际常用血小板膜糖蛋白单免隆抗体（McAb）各类品种对血小板抗原McAb特异性免疫固定检测技术（MAIPA）检测血小板抗体结果的影响。向参加该研究的30个实验室发放10份含已知血小板抗体特异性的血清和1份阴性对照血清样本，以及9份不同类别（GPⅡb／Ⅲa、GPⅠa／，Ⅱa、GPⅠb／Ⅸ及GPⅣ）及克隆编号的血小板膜糖蛋白McAb和统一的实验研究技术方案，各参加实验室按方案进行检测并反馈结果数据，比较同一糖蛋白种类不同的McAb对MAIPA检测结果的影响。结果表明：GPⅡb／Ⅲa中AP2，Gi-5，PL2—73等几种McAb的平均S／CO值较高；GPⅠa／Ⅱa的McAb中MBC202．2和143．1的平均S／CO值较高；GPⅠb／Ⅸ的McAb中142．11和CLB—MB45（C042b）的平均S／CO值较高；GPⅣ的McAb中131．4的平均S／CO值较高。结论不同的McAb品种对样本的检测结果存在差异，McAb对MAIPA的灵敏度有重要影响。用MAIPA法检测血小板抗体时应同时使用相同糖蛋白种类而不同克隆号的McAb，以防止血小板抗体的漏检。

Influence of Different Products of Platelet Membrane Glycoprotein Monoclonal Antibodies Used Internationally on Tests for Monoclonal Antibody-Specific Immobilization of Platelet Antigens

This study was aimed to investigate the influence of different platelet membrane glycoprotein monoclonal antibodies （McAb） which are common used in laboratories on the monoclonal antibody-specific immobilization of platelet antigens （MAIPA） technique according to the request of 14th International Society of Blood Transfusion Platelet Immunology Workshop. 30 participant laboratories were provided with 10 known human platelet antigen （HPA） antibodies, 1 normal serum, 9 different McAbs （ against GP Ⅱ b/Ⅲ a, GP Ⅰ a/Ⅱ a, GP Ⅰ b/Ⅸ and GPⅣ respectively）, and the same protocol. Each participant laboratory carried out the test as the protocol to compare the results of different McAbs against the same glycoprotein and submitted the data to organizer. The results indicated that in McAbs against GP Ⅱ b/Ⅲ a, AP2, Gi-5 and PL2-73 showed higher mean S/CO than that of others ; in GP Ⅰ a/Ⅱ a, MBC202.2 and 143.1 showed higher mean S/CO than that of others; in GP Ⅰ b/Ⅸ, 142.11 and CLB-MB45 （CD42b） showed higher mean S/ CO than that of others; as to GPIV, 131.4 showed higher mean S/CO. In conclusion, capture effects of various McAbs are different, so that different products of McAbs exert influences on the sensitivity of MAIPA. To use a panel of McAbs against the same glycoprotein may avoid the false negative results.