血卟啉单甲醚介导的光动力治疗对巨噬细胞的作用

目的研究血卟啉单甲醚介导的光动力治疗对巨噬细胞的作用。方法检测血卟啉单甲醚随时间增加在巨噬细胞内的聚集量,共聚焦显微镜观察血卟啉单甲醚在细胞的聚集情况;用不同能量对巨噬细胞进行光动力作用,MTT法观察巨噬细胞的存活率,流式细胞仪检测细胞凋亡率。结果血卟啉单甲醚随着孵育时间的增加,细胞内荧光强度增加;随着光动力作用时间的增加细胞活性降低,光动力作用后12 h巨噬细胞的凋亡数量增加,光动力治疗1.5 min组晚期凋亡率(5.82%±3.27%)较对照组(1.20%±3.54%)稍有增加(P<0.05);光动力治疗3min组晚期凋亡率(65.45%±5.17%)较对照组显著增加(P<0.001)。结论血卟啉单甲醚可以在巨噬细胞内聚集,其介导的光动力治疗可以诱导巨噬细胞凋亡。

Hematoporphyrin Monomethyl Ether-Mediated Photodynamic Effects on THP-1 Cell-Derived Macrophages

AimTo investigate the effect of hematoporphyrin monomethyl ether(HMME) mediated photodynamic therapy(PDT) on macrophage.MethodsFluorescence of HMME with different incubated time was tested.Confocal scanning laser microscope was used to investigate HMME subcellular distribution.The survival and apoptosis rate of macrophages were determined by MTT assay and flow cytometer.ResultsHMME was identified to accumulate in the cytoplasm of macrophages by confocal scanning laser microscope.The number of apoptotic macrophages increased slightly in the photodynamic therapy for 1.5 min group(5.82%±3.27%) at 12 h post irradiation(P<0.05),whereas increased significantly in the photodynamic therapy for 3 min group(65.45%±5.17%,P<0.001).ConclusionsHMME could accumulate in macrophages and HMME-mediaded PDT induced macrophage apoptosis.