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| 人参单体皂甙Rh2对前列腺癌细胞株PC-3M细胞移行周期的影响 | |
| 引用本文： | 周桂华,孟艳,李扬,赵雪俭,陈燕萍,马兴元.人参单体皂甙Rh2对前列腺癌细胞株PC-3M细胞移行周期的影响[J].中国病理生理杂志,2002,18(7):823-825. |
| 作者姓名： | 周桂华 孟艳 李扬 赵雪俭 陈燕萍 马兴元 |
| 作者单位： | 1. 吉林大学基础医学院病理生理研究室, 吉林 长春 130021; 2. 吉林大学药学院天然药物化学教研室, 吉林 长春 130021 |
| 摘 要： | 目的:探讨Rh2对体外培养的PC-3M细胞移行周期的影响。方法:应用3H]-TdR掺入法和流式细胞仪测定DNA含量及进行细胞周期的分析。结果:PC-3M细胞的3H]-TdR掺入量明显少于对照组(P＜0.01);流式细胞仪测定G1期细胞数百分比明显高于对照组。结论:Rh2可使PC-3M细胞增殖周期阻滞在G1期,肿瘤细胞增殖减慢。 |
| 关 键 词： | 前列腺肿瘤 人参 细胞周期 |
| 文章编号： | 1000-4718(2002)07-0823-03 |
| 收稿时间： | 2001-02-28 |
| 修稿时间： | 2001年2月28日 |
Effect of ginsenoside Rh2 on cultured PC - 3M cells in vitro |
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| ZHOU Gui-hua ,MENG Yan ,LI Yang ,ZHAO Xue-jian ,CHEN Yan-ping ,MA Xing-yuan.Effect of ginsenoside Rh2 on cultured PC - 3M cells in vitro[J].Chinese Journal of Pathophysiology,2002,18(7):823-825. | |
| Authors: | ZHOU Gui-hua MENG Yan LI Yang ZHAO Xue-jian CHEN Yan-ping MA Xing-yuan |
| Institution: | 1. Laboratory of Pathophysiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China; 2. Department of Natural Pharmaceutical Chemistry, College of Pharmaceutical Sciences, Jilin University, Changchun 130021, China |
| Abstract: | AIM: To explore the influence of ginsenoside Rh 2 on cultured PC-3M cell cycle in vitro . METHODS: DNA content was measured using -TdR incorporation assay. To analysis the cycle changes of PC-3M cells, flow cytometry was used in the experiment.RESULTS: The results indicated that the rate of -TdR incorporation was lower in Rh 2-treated cells than the control in a dose-dependent manner. Flow cytometry demonstrated that the pecent of G 1 phase treated with Rh 2 was higher than that of control. CONCLUSION: These results suggested that PC-3M cells cultured with Rh 2 for 24 h were blocked at G 1 phase, and Rh 2 inhibited the growth of PC-3M cells in vitro . |
| Keywords: | Prostatic neoplasms Ginseng Cell cycle |
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