Plating densities, alpha- difluoromethylornithine effects and time dependence on the proliferation of IEC- 6 cells

Objective To characterize the role of plating densities and alpha- difluoromethylornithine (DFMO) on the proliferation of IEC- 6 cells in vitro.Methods IEC- 6 cells were seeded in 96- well microplates at various densities in the pre sence or absence of DFMO. Cells were counted and their proliferative capabilit y was monitored Days 1 to 7 with MTT assay at an optical density of 570 nm. Results There was a positive relationship between cell number and OD value (r=0.954 , P<0.01). Higher plating densities (>0.5×10(4) cells/well) inhibited the growth of cells on Day 2. When the density reaches 4×10(4) cells/well, the O D value increased gradually and reached a peak on Day 5. After that, the OD va lue began to fall. The growth of IEC- 6 cells was limited at a low density (0. 2×10(4) cells/well) on Day 4. DFMO caused a complete inhibition of proliferati on of IEC- 6 cells on Days 1 to 3. Conclusion Proliferation of IEC- 6 cells is related to plating density and incubation time . It is inhibited by DFMO, but is reversible when the incubation time is prolon ged.