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| 慢性乙型肝炎和肝细胞癌患者乙肝病毒BCP区1762/1764双突变的定量检测及其意义 | |
| 引用本文： | 申悦平,徐君,满晓波,张园海. 慢性乙型肝炎和肝细胞癌患者乙肝病毒BCP区1762/1764双突变的定量检测及其意义[J]. 江苏大学学报(医学版), 2011, 21(6): 514-518 |
| 作者姓名： | 申悦平 徐君 满晓波 张园海 |
| 作者单位： | 1. 镇江市第三人民医院感染科,江苏,镇江,212021 2. 第二军医大学东方肝胆外科医院门诊部,上海,210438 |
| 摘 要： | 目的:观察乙型肝炎病毒基本核心启动子(basic core promoter,BCP)区1762/1764双突变和肝细胞癌发病间的关系及意义.方法:利用已构建的乙肝病毒BCP区A1762T/G1764A双突变复制质粒,设计特异性TaqMan MGB探针,采用特异性探针实时荧光定量PCR(PSRT-PCR)方法对从未进行... |
| 关 键 词： | 肝炎病毒 基本核心启动子 基因突变 乙型肝炎 肝细胞癌 |
Detection and significance of double mutation in basic core promoter in hepatitis B virus in chronic hepatitis B and hepatocellular carcinoma |
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| SHEN Yue-ping,XU Jun,MAN Xiao-bo,ZHANG Yuan-hai. Detection and significance of double mutation in basic core promoter in hepatitis B virus in chronic hepatitis B and hepatocellular carcinoma[J]. Journal of Jiangsu University Medicine Edition, 2011, 21(6): 514-518 | |
| Authors: | SHEN Yue-ping XU Jun MAN Xiao-bo ZHANG Yuan-hai |
| Affiliation: | 1.Department of Infectious Disease,the Third People′s Hospital of Zhenjiang,Zhenjiang Jiangsu 212021;2.Department of Outpatient,Eastern Hepatobiliary Surgery Hospital of Second Military Medical University,Shanghai 210438,China) |
| Abstract: | Objective: To explore the feasibility of quantitative detection of double mutation in basic core promoter(BCP) 1762/1764 sites in hepatitis B virus(HBV) in chronic hepatitis B(CHB) and hepatocellular carcinoma(HCC) and the relationship between the mutation and HCC and its clinical significance.Methods: The mutant was accurately detected using probe-specific real-time quantitative polymerase chain reaction(PSRT-PCR) which adopted TaqMan MGB probe,the designing was depended on the double mutation plasmids whi... |
| Keywords: | hepatitis B virus basic core promoter mutation hepatitis B hepatocellular carcinoma |
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