Production of monoclonal antibodies for detection of antigens in both their native and denatured states |
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| Authors: | Vibeke Barkholt Kirsten Ebbeh⊘j Susanne S⊘rensen Charlotte Arentsen Maria Dahl Hanne Fr⊘klær |
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| Affiliation: | Department of Biochemistry and Nutrition , Technical University of Denmark , Building 224, Lyngby, DK 2800, Denmark |
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| Abstract: | Different immunization procedures were tested to find methods to enhance the proportion of monoclonal antibodies reacting equally well with native and denatured proteins for use in food analysis. Antibodies to soybean trypsin inhibitor with the desired characteristics were obtained by immunization with the denatured protein. Antibodies to ovomucoid were obtained by long‐term immunization. The monoclonal antibodies to ovomucoid were of the IgM class. |
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| Keywords: | Food analyses denatured components monoclonal antibodies immunization procedures |
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