| 制何首乌多糖的纯度鉴定和理化性质研究 |
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| 引用本文： | 蒋秀红,王霆,崔永霞,梁生旺,申洪超. 制何首乌多糖的纯度鉴定和理化性质研究[J]. 中国现代药物应用, 2009, 3(24): 6-7 |
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| 作者姓名： | 蒋秀红 王霆 崔永霞 梁生旺 申洪超 |
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| 作者单位： | 1. 河南中医学院,450008
2. 广东药学院 |
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| 摘 要： | 目的对制何首乌多糖进行纯度鉴定和理化性质研究。方法采用水提醇沉法提取制何首乌多糖，经过纤维素和凝胶柱层析纯化后，纯化后的多糖PRPM-1、PRPM-2和PRPM-3分别用Sepha-dexG-100柱层析和紫外吸收光谱检测其纯度；通过物理和化学方法分析多糖理化性质。结果制何首乌多糖为均一多糖。结论该测定方法准确，重复性好。
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| 关 键 词： | 制何首乌 多糖 纯度鉴定 理化性质 |
Study on purity identification and physicochemical characteristics of polysaccharide from radix polygoni multiflori preparata |
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| Affiliation: | JIANG Xiu-hong, WANG Ting, CUI Yong-xia, et al( Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China ) |
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| Abstract: | Objective To Study on purity identification and physicoehemical characteristics of polysaccharide from radix polygoni multiflori preparata. Method A polysaccharide was obtained from Radix Polygoni Multiflori Preparata by method of water extraction and ethanol precipitation protein ; the polysaeeharide was separated with DEAE-52 column and Sephadex G-100 column; the result indicated that PRPM-1, PRPM-2 and PRPM-3 was homogeneous molecular weight assayed by UV scanning paaern and Sephadex G-100 column chromatography. Result The results showed PRPM-1, PRPM-2 and PRPM-3 were homogeneous polysaccharides. Conclusion The method of determination is reliable and has good repetitions. |
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| Keywords: | Radix polygoni muhiflori preparata Polysaccharide Purity identification Physicochemical characteristics |
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