重症肌无力患者胸腺自身免疫相关基因表达的研究

目的探讨MG患者和正常对照者的胸腺组织炎症反应与自身免疫性疾病相关基因的表达情况。方法取MG患者（MG组）和正常对照者（对照组）的胸腺组织标本，提取总RNA，反转录合成cDNA后，体外转录合成生物素标记的cRNA与基因芯片进行杂交，通过化学发光法检测基因表达，比较两组胸腺组织基因表达的差异。结果MG组与对照组比较，有61条基因存在显著性差异，其中表达上调33条，下调28条，这些基因包括炎症相关趋化因子基因、细胞因子及其受体基因、与细胞因子产生与代谢有关的基因、细胞因子及其受体相互作用相关的基因、炎症反应相关基因以及体液免疫相关基因等。结论芯片筛选MG患者胸腺自身免疫相关基因表达与正常对照组存在显著差异。

Exploration of differential gene status in thymus of myasthenia gravis patients by genechip

Objective To explore the differential expression of autoimmune and inflammatory response gene in thymus between myasthenia gravis patients and healthy controls.Methods The total RNA was extracted from thymus of myasthenia gravis and healthy controls,and reversely transcribed in cDNA templates to synthesize cRNA probes labeled for hybridization with the oligo autoimmune and inflammatory response gene array to explore the differencially expressed gene.Results Among 440 genes there were 61 differences,of which...