高效液相色谱法测定减肥类保健食品中7种功效成分

目的建立减肥类保健食品7种功效成分(左旋肉碱、L-茶氨酸、儿茶素、吡啶甲酸铬、番泻苷A、番泻苷B、鼠尾草酸)的高效液相色谱测定方法。方法样品直接用50%乙腈超声提取,采用C18色谱柱分离,以甲醇-磷酸二氢钾(0.1%磷酸)水溶液为流动相,梯度洗脱,外标法定量。结果左旋肉碱、L-茶氨酸和鼠尾草酸在10.0~100.0 mg/L,儿茶素在1.0~50.0 mg/L,番泻苷A在0.50~10.0 mg/L,番泻苷B在0.20~10.0 mg/L,吡啶甲酸铬在2.0~50.0 mg/L内质量浓度和色谱峰面积有良好的线性关系,相关系数r≥0.9991。方法回收率为89.9%~108.0%,相对标准偏差为1.2%~6.3%,方法检出限为0.1~5 mg/kg。结论该方法快速,准确,灵敏,适合减肥类保健食品中7种功效成分的同时测定。

Determination of 7 kinds of functional compositions in slimming health food by high performance liquid chromatography

Objective To develop an analytical method based on high performance liquid chromatography (HPLC) for determination of 7 kinds of functional compositions (including L-carnitine, L-theanine, catechin, chromium picolinate, sennoside A, sennoside B and carnosic acid) in slimming health food. Methods Samples were extracted by 50% acetonitrile. The chromatographic separation was performed on C18 column using a mobile phase consisting of methanol and potassium dihydrogen phosphate solution (0.1% phosphoric acid) under gradient mode. And external standard method was used for quantitative analysis.Results A good linearity was got in the range of 10.0 mg/L-100.0 mg/L for L-carnitine, L-theanine and carnosic acid, in the range of 1.0 mg/L-50.0 mg/L for catechin, in the range of 0.50 mg/L-10.0 mg/L for sennoside A, in the range of 0.20 mg/L-10.0 mg/L for sennoside B, and in the range of 2.0 mg/L-50.0 mg/L for chromium picolinate. The correlation coefficients were equal to or greater than 0.9991. The recoveries were in the range of 89.9%-108.0%, the relative standard deviation was between 1.2% and 6.3%, and the limits of detection were 0.1 mg/kg-5 mg/kg. Conclusions This method is rapid, accurate and feasible, and it is suitable for simultaneous quantification of 7 kinds of functional compositions in slimming health food.