STIP1在脑胶质瘤组织中的表达及对胶质瘤细胞株增殖、凋亡、侵袭的影响

目的:检测磷酸化应激诱导蛋白1（stress-induced phosphoprotein 1，STIP1）在胶质瘤组织和正常脑组织中的表达差异，探讨STIP1对胶质瘤细胞株U87、U251细胞增殖、凋亡及侵袭能力的影响。方法:运用实时定量聚合酶链式反应(qRT-PCR)、免疫组化及蛋白印迹检测胶质瘤组织和正常脑组织中STIP1的表达；小干扰RNA（STIP1-siRNA）转染U87、U251细胞株后，使用噻唑蓝(MTT)法、流式细胞仪和Transwell小室观察细胞的增殖、凋亡和侵袭能力。结果:STIP1在胶质瘤Ⅳ级组织中的表达明显高于正常脑组织，但在胶质瘤Ⅱ、Ⅲ级中的表达与正常脑组织无明显差异。U87、U251细胞株转染STIP1-siRNA后，细胞增殖和侵袭能力明显受到抑制，而细胞凋亡率明显上升。结论:STIP1在胶质母细胞瘤组织中高表达，下调STIP1可以抑制胶质瘤细胞株U87和U251的增殖，阻滞细胞周期，促进其细胞凋亡，并可抑制其侵袭力。

Expression of STIP1 in glioma tissues and its effect on proliferation，apoptosis and invasion of glioma cell lines

Objective:To investigate the expression difference of stress-induced phosphoprotein 1（STIP1）in glioma tissues and normal brain tissues，and its effects on proliferation，apoptosis and invasion of glioma cell lines (U87 and U251).Methods:Real-time quantitative polymerase chain reaction (qRT-PCR)，immunohistochemistry，and Western blot were used to detect the expression of STIP1 in glioma tissues and normal brain tissues.Small interfering RNA（STIP1-siRNA）was used to transfect U87 and U251 cell lines.Then the effects of cell proliferation，apoptosis and invasion were observed by MTT method，flow cytometry and Transwell chamber.Results:The expression of STIP1 in glioma grade Ⅳ tissues was significantly higher than in non-tumor brain tissues，but it was not significantly different between glioma grade Ⅱ and Ⅲ and non-tumor brain tissues.After U87 and U251 cell lines were transfected with STIP1-siRNA，the cell proliferation and invasion ability were significantly inhibited，and the apoptosis rate was increased significantly.Conclusion:STIP1 is highly expressed in glioblastoma tissues.Down-regulating STIP1 can inhibit the proliferation of glioma cell lines U87 and U251，block the cell cycle，promote its apoptosis，and inhibit its invasiveness.