miR-147对胶质瘤U87细胞增殖、凋亡和侵袭的影响

目的:比较胶质瘤患者胶质瘤组织和瘤旁组织中miR-147表达水平的差异性，进一步分析miR-147与胶质瘤细胞生物学活性的相关性。方法:通过实时荧光定量PCR检测65例胶质瘤患者瘤组织和瘤旁组织中miR-147的表达水平；采用Lipofectamine 2000转染法转染胶质瘤细胞，分为miR-147 mimics组和NC组，检测两组转染效率；进一步通过CCK-8实验、流式细胞实验和Transwell实验比较两组细胞生物活性。结果:胶质瘤组织中miR-147表达水平显著低于瘤旁组织；与NC组比较，miR-147 mimics组细胞不论是增殖能力还是侵袭能力都明显降低，但是，miR-147 mimics组的细胞凋亡率升高。结论:胶质瘤组织中存在miR-147低表达的现象。miR-147在胶质瘤细胞中表达升高能够抑制细胞的增殖能力、降低其侵袭能力，并促使更多的细胞发生晚期凋亡。miR-147可能在胶质瘤中扮演着抑癌基因的重要角色。

Study on the correlation between miR-147 and biological activity of U87 cells in glioma cells

Objective:To compare the difference of miR-147 expression between glioma tissues and adjacent tissues，and to further analyze the correlation between miR-147 and biological activity of glioma cells.Methods:The expression of miR-147 in 65 glioma tissues and adjacent tissues was detected by real-time fluorescence quantitative PCR，and the glioma cells were transfected into miR-147 mimics group and NC group by Lipofectamine 2000 method，and the transfection efficiency of the two groups was detected.Furthermore，CCK-8 assay，flow cytometry test and Transwell assay were used to compare the cell biological activity between the two groups.Results:The expression level of miR-147 in glioma tissue was significantly lower than that in paratumor tissue，and compared with NC group，the proliferation and invasion ability of miR-147 mimics group were decreased，but the apoptosis rate was increased.Conclusion:There is a low expression of miR-147 in glioma tissues.Overexpression of miR-147 can inhibit the proliferation and invasion of glioma cells and induce apoptosis of glioma cells，which plays an anti-cancer role.