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| 高效液相色谱法测定苦参中红车轴草苷的含量 | |
| 引用本文： | 赵月珍,向大雄. 高效液相色谱法测定苦参中红车轴草苷的含量[J]. 中南药学, 2008, 6(1): 50-52 |
| 作者姓名： | 赵月珍 向大雄 |
| 作者单位： | 1. 中南大学药学院,长沙,410013;湖南中医药高等专科学校,湖南,株洲,412012 2. 中南大学药学院,长沙,410013 |
| 摘 要： | 目的建立测定苦参中红车轴草苷含量的高效液相色谱方法。方法HYPERSIL BDS C18色谱柱（4．6mm×200mm，5μm），流动相为甲醇-0．2％磷酸水溶液梯度洗脱（MeOH％：0 min10％，20min40％），流速为1．0mL·min^-1，检测波长为310nm，柱温为25℃。结果红车轴草苷在0．446～8．920／μg线性良好，相关系数r为0．9999，平均回收率为97．94％，RSD＜1％（n＝6）。结论本方法操作简便，分离效果好，灵敏度高，重现性好，为控制苦参药材质量提供参考。 |
| 关 键 词： | 红车轴草苷 苦参 高效液相色谱法 含量测定 |
| 文章编号： | 1672-2981（2008）01-0050-03 |
| 收稿时间： | 2007-11-20 |
| 修稿时间： | 2007-12-19 |
Determination of trifolirhizin in Sophora flavescens Ait. by HPLC |
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| ZHAO Yue-zhen,XIANG Da-xiong. Determination of trifolirhizin in Sophora flavescens Ait. by HPLC[J]. Central South Pharmacy, 2008, 6(1): 50-52 | |
| Authors: | ZHAO Yue-zhen XIANG Da-xiong |
| Affiliation: | ZHAO Yue-zhen, XIANG Da xiong. (1. School of Pharmaceutical Sciences, Central South University, Chang-sha 410013; 2. Hunan Traditional Chinese Medical College, Zhuzhou Hunan 412012) |
| Abstract: | Objective To established an HPLC method for the determination of trifolirhizin in Sophora flavescens Air. Methods A column of HYPERSIL BDS C18 （4.6 mm× 200 mm, 5/μm） was used. The mobile phase was composed of methanol and 0.2% phosphoric acid with a linear gradient elution from 10%to 40%methanol in 20 minutes. The flow rate was 1.0 mL·min^-1 , and the detection wavelength was 310 nm. Results The calibration curve of trifolirhizin was in good linearity over the range of 0. 446～8. 920 μg , （r=0. 999 9）. The average recovery was 97.94%, and RSD was less than 1%（n=6）. Conclusion The method is simple, sensitive, accurate, reproducible, and can be used to control the quality of Sophora flavescens Air. |
| Keywords: | trifolirhizin Sophora flavescens Ait HPLC content determination |
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