| 人鼻咽与鼻咽癌cDNA阵谱中DNA修复相关基因表达差异的初步研究 |
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| 引用本文： | Han W,Li H,Xie L,Xu L,Zhang L,Yao K. 人鼻咽与鼻咽癌cDNA阵谱中DNA修复相关基因表达差异的初步研究[J]. 中华肿瘤杂志, 2002, 24(2): 114-117 |
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| 作者姓名： | Han W Li H Xie L Xu L Zhang L Yao K |
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| 作者单位： | 410078,长沙,中南大学湘雅医学院教育部癌变和侵袭重点实验室 |
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| 基金项目： | 国家自然科学基金重点资助项目 (3 973 0 2 0 0 ),中华医学会基金重点资助项目 (96 65 5 ) |
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| 摘 要： | 目的 用cDNA阵卤咽癌组织及正常组织的基因表达谱,研究鼻咽癌组织内修复相关基因的表达差异。方法 Atlas human cancer cDNA expression array 7742-1杂交后,用Atlas Image 1.01a分析滤膜杂交结果,RT－PCR反应验证滤膜杂交结果。结果 在588个肿瘤相关基因中,共有134个基因表达上调,88个基因表达下调。在有DNA损伤应答、修复、重组相关基因的C区中,表达上调的基因有21个,与修复相关的有6个;表达下调的有44个,与修复相关的则有12个。结论 DNA修复相关基因的改变可能在鼻咽癌病理生理过程中起一定作用。
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| 关 键 词： | 鼻咽肿瘤 cDNA阵谱 DNA修复相关基因 基因表达 鼻咽癌 |
| 修稿时间： | 2001-07-30 |
Plerosis of cDNA array of normal human nasopharyngeal tissue and nasopharyngeal carcinoma |
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| Han Weinong,Li Hong,Xie Lu,Xu Liangguo,Zhang Ling,Yao Kaitai. Plerosis of cDNA array of normal human nasopharyngeal tissue and nasopharyngeal carcinoma[J]. Chinese Journal of Oncology, 2002, 24(2): 114-117 |
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| Authors: | Han Weinong Li Hong Xie Lu Xu Liangguo Zhang Ling Yao Kaitai |
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| Affiliation: | Laboratory of Cancerous Change and Invasion, Xiangya Medical College, Central South University, Changsha 410078, China. |
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| Abstract: | OBJECTIVE: To compare gene expression gene profile of nasopharyngeal carcinoma (NPC) tissue with that of normal nasopharyngeal tissues by cDNA array and to discuss possible functions of DNA repair-related genes in NPC tissue. METHODS: After hybridization of atlas human cancer cDNA expression array 7742 - 1, atlas hybridization results were analyzed by Atlas Image 1.01 a software package. Using RT-PCR was used to confirm the results. RESULTS: Of 63 differentially expressed genes in quadrangle C including DNA damage response, repair & recombination-related genes, 6 DNA repair-related genes were up-regulated, 12 were down-regulated. CONCLUSION: DNA repair-related genes may be involved in patho-physiological process of nasopharyngeal carcinoma. |
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| Keywords: | Nasopharyngeal neoplasms Nasopharynx cDNA Array DNA repair related genes |
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