| 高效液相色谱法测定维生素C注射液含量 |
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| 引用本文： | 刘国如,刘永锁,陈振玲,王伟,丰廷宗,周毓瑾.高效液相色谱法测定维生素C注射液含量[J].中国医药导报,2011,8(27):50-51. |
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| 作者姓名： | 刘国如 刘永锁 陈振玲 王伟 丰廷宗 周毓瑾 |
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| 作者单位： | 中国民用航空局民用航空医学中心(民航总医院),北京,100023 |
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| 摘 要： | 目的：建立高效液相色谱法测定维生素C注射液中维生素C含量的方法。方法：采用Phenomenex luna-NH2分析色谱柱（4.6 mm×250 mm,5μm）,流动相为磷酸二氢钾缓冲液-乙腈（1∶1,磷酸调至pH 3.0）;流速：1.0 ml/min;检测波长：245 nm。结果：维生素C的线性回归方程为Y=29 560X-21 310（r=0.999 5）,线性范围为5～30μg/ml,平均回收率为98.30%（n=9,RSD为2.1%）。结论：该方法操作简便,结果准确,可用于测定维生素C注射液中维生素C的含量。
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| 关 键 词： | 高效液相色谱法 维生素C注射液 维生素C 含量测定 |
Determination of Vitamin C Injection by HPLC |
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| LIU Guoru,LIU Yongsuo,CHEN Zhenling,WANG Wei,FENG Tingzong,ZHOU Yujin.Determination of Vitamin C Injection by HPLC[J].China Medical Herald,2011,8(27):50-51. |
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| Authors: | LIU Guoru LIU Yongsuo CHEN Zhenling WANG Wei FENG Tingzong ZHOU Yujin |
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| Institution: | LIU Guoru,LIU Yongsuo,CHEN Zhenling,WANG Wei,FENG Tingzong,ZHOU Yujin Civil Aviation Medicine Centre(Civil Aviation General Hospital),CAAC,Beijing 100023,China |
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| Abstract: | Objective： To develop a quantitative method for the determination of Vitamin C in Vitamin C Injection.Methods： Phenomenex luna-NH2 column（4.6 mm×250 mm,5 μm） was used as chromatographic column,a mixture of acetonitrile and dibasic potassium phosphate buffer solution（1∶1,v/v） was used as mobile phase,which was adjusted to pH 3.0 by phosphoric acid,and the detection wavelength was at 245 nm with a flow rate of 1.0 ml/min.Results： The regression equation was Y=29 560X-21 310（r=0.999 5）,which was linear over a range from 5 μg/ml to 30 μg/ml.The average recovery was 98.30%（n=9,RSD=2.1%）.Conclusion： This method is sensitive,rapid and accurate,which is can be used for determination of Vitamin C in Vitamin C Injection. |
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| Keywords: | HPLC Vitamin C Injection Vitamin C Determination |
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