RGDS四肽促进大鼠肝星状细胞胶原降解机制的研究

目的：观察精-甘-天冬-丝氨酸（RGDS）四肽对纤维连接蛋白（FN）刺激的大鼠肝星状细胞（HSC）基质金属蛋白酶-13（MMP-13）及其组织抑制因子（TIMP-1）表达的影响。方法：应用体外细胞培养技术，用RT-PCR检测HSC MMP-13mRNA水平；原位杂交和Western blot技术分别检测上述细胞TIMP-1 mRNA和蛋白水平。结果：RGDS四肽干预2h组MMP-13 mRNA的表达强度明显上调，同时TIMP-1 mRNA表达受抑制；RGDS四肽干预24h组TIMP-1蛋白表达受抑制。结论：RGDS四肽诱导HSC MMP-13表达，抑制其TIMP-1表达是其抗肝纤维化的作用机制之一。

The degrading mechanism of collagen promoted by RGDS in hepatic stellate cell

AIM: To study the effect of Arg-Gly-Asp-Ser (RGDS) tetrapeptide on the expression of matrix metalloproteinase-13 (MMP-13) and the tissue inhibitor of metalloproteinase-1(TIMP-1) in hepatic stellate cell(HSC) stimulated by fibronectin (FN). METHODS: The expression of MMP-13 was evaluated by RT-PCR. TIMP-1 was analyzed by in-situ hybridization at mRNA level and by Western blot at protein level. RESULTS: Treated by RGDS tetrapeptide for 2 h, the mRNA expression of MMP-13 was upregulated but the expression of TIMP-1 mRNA and its protein in HSC was inhibited. CONCLUSION: The expression of MMP-13 can be induced by RGDS tetrapeptide and the expression of TIMP-1 can be inhibited by RGDS tetrapeptide, which is one of the mechanisms of its anti-fibrogenesis.