八肽胆囊收缩素对脂多糖诱导离体兔胸主动脉反应性改变的影响

目的和方法:应用离体血管环张力测定技术,观察八肽胆囊收缩素(CCK-8)对脂多糖(LPS)诱导兔离体胸主动脉反应性变化的影响。在扫描电镜下观察内皮细胞超微结构的变化,以探讨CCK-8抗内毒素休克作用的可能机制。结果:LPS(100mg/L)孵育后的胸主动脉环对乙酰胆碱(ACh)的内皮依赖性舒张反应降低,且呈时间依赖性;对苯肾上腺素(PE)的收缩反应降低;孵育7h血管内皮细胞结构损伤;CCK-8(1mg/L)可减轻LPS的损伤效应;CCK-8(1mg/L)单独孵育对胸主动脉的舒缩反应及内皮细胞的超微结构无明显影响。结论:CCK-8能减轻LPS诱导兔离体胸主动脉反应性的异常改变,这可能是CCK-8抗内毒素休克作用的机制之一。

Effects of cholecystokinin octapeptide on changes in rabbit thoracic aortic reactivities induced by lipopolysaccharides in vitro

AIM and METHODS: To elucidate the mechanism of anti-endotoxic shock of cholecystokinin octapeptide(CCK-8), the effects of CCK-8 on changes in rabbit thoracic aortic reactivities induced by lipopolysaccharides(LPS) in vitro were studied, and the ultrastructure of the endothelial cells was observed under scanning electron microscope. RESULTS: Incubation of thoracic aortic rings(TARs) with LPS(100 mg/L) resulted in an time-dependent impairment of the endothelium-dependent relaxations to acetylcholine(incubation for 3, 7, 14 h), a reduction of contractive response to phenylphrine(incubation for 14 h) and ultrastructural injury in endothelial cells(incubation for 7 h), all of which were alleviated by concomitant incubation with CCK-8(1 mg/L). In contrast, neither the vascular contractions nor the relaxations were affected by CCK-8 (1 mg/L) alone. CONCLUSION: CCK-8 improved the vascular reactivities in the presence of LPS, which may be one of the anti-endotoxic shock mechanisms of CCK.