复方甘草酸苷对体外培养的角质形成细胞的作用

目的观察复方甘草酸苷(CG)对角质形成细胞表达CXCR2及分泌TGF-β1的影响。方法不同浓度CG处理人角质形成细胞株HaCat,MTT法检测CG对HaCat的增殖抑制作用,流式细胞仪分析CG处理后的HaCat细胞的CXCR2表达。培养正常人表皮角质形成细胞,观察CG处理后,培养液中TGF-β1含量的变化。结果50~200μl/m l的CG抑制HaCat细胞的增殖,干预24 h即可见到明显的抑制作用,CXCR2的表达明显低于正常对照组。正常原代角质形成细胞的培养上清中TGF-β1分泌增加。结论50~200μl/m l CG促进素角质形成细胞表达CXCR2和TGF-β1分泌,抑制角质形成细胞的增殖。

The Effect of Complex Glycyrrhizin on the Keratinocytes Cultured in Vitro

Objective To investigate the effect of complex glycyrrhizin(CG) on the CXCR2 expression and TGF-β1 secretion content in keratinocytes cultured in vitro.Methods The keratinocyte cell line HaCat was cultured with CG at various concentration,the cells proliferation were detected by MTT method.The expression of CXCR2 was assayed by flow cytometry.The secretion content of TGF-β1 in the culture of keratinocyte from normal human epidermis was examined by ELISA.Results CG at concentrations between 50~200 μl/ml could obviously inhibited proliferation of keratinocyte,and the obvious decrease could be detected after 24 h treated with CG.The expression of CXCR2 in HaCaT cells was also slightly up-regulated.At the same time,the secretion of TGF-β1 in culture of normal human keratinocyte was enhanced by adding CG into media.Conclusion CG could suppress cultured keratinocyte proliferation by regulating expression of CXCR2 and enhancing secretion of TGF-β1.