酚妥拉明和地塞米松对吸入性损伤大鼠肾脏损伤的保护作用及其机制

目的：观察酚妥拉明和地塞米松重度吸入性损伤大鼠肾脏损害的保护作用及其机制。方法：７２只大鼠随机分为３组,对照组腹腔注射重盐水,Ａ组腹腔注射酚妥拉明,Ｂ组腹腔注射地塞米松,另取６只非致伤大鼠作伤前值测定,再取６只致伤大鼠,伤后３０分名贵作伤后值测定。结果：对照组尿素氮（ＢＵＮ）,再二醛（ＭＤＡ）、ＭＤＡ／超氧化物歧化酶（ＳＯＤ）及组织含水量增增高,血中ＮＯ水平升高,肾组织中ＮＯ水平变化不如血中明显,

The effect of phentolamine and dexamethasone on renal injury in rats with severe smoke inhalation injury

ObjectiveTo observe the protective effect of phentolamine and dexamethasone on renal injury in rats with severe smoke inhalation injury,and to explore their potential mechanism.Methods72 rats were divided into three groupscontrol group (saline treatment),phentolamine treatment group (group A),and dexamethasone treatment group (group B).In addition,6 animals without inhalation injury served as normal controls,and another 6 animals subjected to inhalation injury were sacrificed at 30 minutes.ResultsIn the control group,levels of blood urea nitrogen (BUN),malondialdehyde (MDA),MDA/superoxide dismutase (SOD),and tissue water content increased,and plasma NO levels increased while renal SOD,ATP contents obviously decreased.Blood BUN and plasma and tissue nitric oxide (NO) levels in both group A and group B were markedly lower than those in control group.Contents of SOD and ATP in both groups were higher compared with the controls,together with decrease in tissue water content in two treatment groups.ConclusionsBoth phentolamine and dexamethasone can regulate renal NO level through improvement of antioxidation ability and energy metabolism,which may be associated with the protective effect on the renal function in rats with severe smoke inhalation injury.