| 维生素D3对黑色素瘤细胞株生长及凋亡的影响 |
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| 引用本文： | 郑天虎,贺岩,李莹杰. 维生素D3对黑色素瘤细胞株生长及凋亡的影响[J]. 实用肿瘤学杂志, 2006, 20(1): 27-29 |
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| 作者姓名： | 郑天虎 贺岩 李莹杰 |
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| 作者单位： | 哈尔滨医科大学生化教研室,哈尔滨,150081%哈尔滨医科大学病理教研室 |
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| 摘 要： | 目的研究维生素D3对黑色素瘤细胞株A375生长及凋亡的影响.方法采用四唑氮蓝比色(MTT)法检测细胞增殖,光镜观察细胞形态,流式细胞仪测定细胞周期和凋亡率,末端脱氧核苷酸转移酶介导的原位酶标记(TUNEL)法计数凋亡细胞.结果10-7mol/L的1.25(OH)2D3可以抑制A375增生,改变细胞周期时相分布,致G0/G1期阻滞,加强细胞毒性化疗药物紫杉醇的作用,促进细胞凋亡.结论维生素D3可以作为细胞凋亡诱导剂,成为新一种黑色素肿瘤治疗药物.
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| 关 键 词： | 黑色素瘤细胞 维生素D3 紫杉醇 细胞凋亡 |
| 收稿时间： | 2005-12-16 |
| 修稿时间： | 2005-12-16 |
The effect of vitamin D3 on growth and apoptosis in melanoma cell line A375 |
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| ZHENG Tianhu,HE Yan,LI Yingjie. The effect of vitamin D3 on growth and apoptosis in melanoma cell line A375[J]. Journal of Practical Oncology, 2006, 20(1): 27-29 |
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| Authors: | ZHENG Tianhu HE Yan LI Yingjie |
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| Affiliation: | 1. Department of Biochemistry, Harbin Medical University, Harbin 150081; 2. Department of pathology, Harbin Medical University |
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| Abstract: | Objective To study the effect of vitamin D_3 on growth and apoptosis in melanoma cell A375.Methods We compared cell numbers by using MTT method,analyzed cell cycle and apoptosis percentage by flow cytometre.Observed cellular structure with microscope,determined quantitatively apoptosis cells by terminal deoxynucleotidy transferase-mediated dUTP nick end labeling(TUNEL).Results Incubation with vitamin D_3 10~(-7) mol/L,A375 cells exhibited significant growth inhibition.Flow cytometric analysis indicted cell G0/G1 arrest along with increasing apoptotic peak and percentage.The action of cytotoxic drug taxol was strengthened.Conclusion Vitamin D_3 could be a new drug for the treatment of melanoma. |
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| Keywords: | Melanoma Vitamin D_3 Apotosis Taxol(Paclitaxel) |
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